This country code will remain if no action is taken to change it.
Our website uses functional cookies that do not collect any personal information or track your browsing activity. When you select your country, you agree that we can place these functional cookies on your device.
JM109 is a K strain that is recA– and endA– to minimize recombination and improve the quality of plasmid DNA. In addition, the cells carry the F´ episome, which allows blue/white screening. JM109 Competent Cells are available for convenient transformation in two efficiencies: High Efficiency at greater than 108cfu/μg and Subcloning Efficiency at greater than 107cfu/μg.
HB101 cells are recA–, which minimizes recombination events, and contain a restriction-minus background (hsdS20) that allows better representation when cloning methylated DNA. HB101 Competent Cells are suitable fo...
Expand to Read More »
HB101 cells are recA–, which minimizes recombination events, and contain a restriction-minus background (hsdS20) that allows better representation when cloning methylated DNA. HB101 Competent Cells are suitable for vectors that do not require α-complementation for blue/white screening.
For researchers doing more than one transformation, competent cells are available in standard format (200µl aliquots). For added convenience, single-use competent cells (50µl aliquots) also are offered.
JM109 and HB101 Competent Cells are prepared according to a modified procedure of Hanahan.
JM109 Genotype: endA1, recA1, gyrA96, thi, hsdR17 (rk–, mk+), relA1, supE44, Δ( lac-proAB), [F´ traD36, proAB, laqIqZΔM15].
HB101 Genotype: F–, thi-1, hsdS20 (rB–, mB–), supE44, recA13, ara-14, leuB6, proA2, lacY1, galK2, rpsL20 (strr), xyl-5, mtl-1.
For more information, see the Protocols & Applications Guide.
« Collapse Content
Single-Use JM109 Competent Cells, >108cfu/µg
JM109 Competent Cells, >107cfu/μg
JM109 Competent Cells, Subcloning Efficiency
Competent Cells Control DNA
JM109 Competent Cells, >108cfu/μg
JM109 Competent Cells, High Efficiency
Single-Use HB101 Competent Cells, >108cfu/µg
HB101 Competent Cells, >108cfu/μg
HB101 Competent Cells, High Efficiency
Single-Use Pro 5-alpha Competent Cells, >109cfu/µg
Single-Use Pro 5-α Competent Cells, >109cfu/µg
Store at –70°C.
For product intended use please see Patents & Disclaimers tab.
L2005, L1001, L2001, L2015, L2011, L1221 For Research Use Only. Not for Use in Diagnostic Procedures.
View All Reviews »
Review This Product »
We offer a range of services to help you succeed using Promega technologies. From product training to set up of automated systems and development of custom applications—our scientific support goes beyond the basics.
Ask us! We are here to help you.