JM109 and HB101 Competent Cells are prepared according to a modified procedure of Hanahan. JM109 Competent Cells are available for convenient transformation in two efficiencies: High Efficiency at greater than 108cfu/μg and Subcloning Efficiency at greater than 107cfu/μg. JM109 cells are an ideal host for many molecular biology applications. HB101 cells are useful for cloning in vectors that do not require α-complementation for blue/white screening. pGEM®-3Z Vector is provided as a positive control.
JM109 Genotype: endA1, recA1, gyrA96, thi, hsdR17 (rk–, mk+), relA1, supE44, Δ( lac-proAB), [F´ traD36, proAB, la...
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JM109 and HB101 Competent Cells are prepared according to a modified procedure of Hanahan. JM109 Competent Cells are available for convenient transformation in two efficiencies: High Efficiency at greater than 108cfu/μg and Subcloning Efficiency at greater than 107cfu/μg. JM109 cells are an ideal host for many molecular biology applications. HB101 cells are useful for cloning in vectors that do not require α-complementation for blue/white screening. pGEM®-3Z Vector is provided as a positive control.
JM109 Genotype: endA1, recA1, gyrA96, thi, hsdR17 (rk–, mk+), relA1, supE44, Δ( lac-proAB), [F´ traD36, proAB, laqIqZΔM15].
HB101 Genotype: F–, thi-1, hsdS20 (rB–, mB–), supE44, recA13, ara-14, leuB6, proA2, lacY1, galK2, rpsL20 (strr), xyl-5, mtl-1.
Features - Benefits
- Convenient: Ready to use; no preparation time necessary.
- Reliable: Transformation efficiencies guaranteed; positive control included.
- Versatile: Use JM109 for subcloning, T-vector cloning, blue/white screening, production of single-stranded DNA from phagemids (F´ episome).
- High Transformation Efficiency: Greater than 108cfu/μg (Cat.# L2001 and L2011).
- Safe: The recA– mutation prevents undesirable recombination events, and the endA– mutation in JM109 cells prevents carryover nuclease in miniprep DNA.
Applications
For more information, see the Protocols & Applications Guide.
References
- Hanahan, D. (1985) DNA Cloning, Vol. 1, Glover, D., ed., IRL Press, Ltd., 109.
- Yanisch-Perron, C. et al. (1985) Gene 33, 103–19.
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