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Promega Corporation

Bacterial Strains BMH 71-18 mutS and ES1301 mutS

BMH 71-18 mutS and ES1301 mutS are mismatch repair minus strains of E. coli. Use of these strains prevents repair of the newly synthesized unmethylated strand, leading to high mutation efficiencies. Both ES1301 and BMH 71-18 mutS are recA+, therefore inserts containing highly repetitive sequences may be unstable. ES1301 is Tets, which allows for cycling between antibiotic resistances. BMH 71-18 is Tetr; therefore, it can only be used to select Ampr or CATr mutants.

ES1301 mutS Genotype: lacZ53, mutS201::Tn5, thyA36, rha-5, metB1, deoC, IN(rrnD–rrnE).

BMH 71-18 mutS Genotype: thi, supE, Δ(lac-proAB), [mutS::T...

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  • *Prices valid for US customers only
Product Size Conc. Catalog # *List Price QTY Add to Cart

Bacterial Strain ES1301 mutS, Glycerol Stock (noncompetent)

- Q6131 $ 47.00 Add to cart

Bacterial Strain BMH 71-18 mutS, Glycerol Stock (noncompetent)

- Q6321 $ 62.00 Add to cart

Storage Conditions

Store at –70°C.

For product intended use please see Patents & Disclaimers tab.

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