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Bacterial Strains BMH 71-18 mutS and ES1301 mutS

BMH 71-18 mutS and ES1301 mutS are mismatch repair minus strains of E. coli. Use of these strains prevents repair of the newly synthesized unmethylated strand, leading to high mutation efficiencies. Both ES1301 and BMH 71-18 mutS are recA+, therefore inserts containing highly repetitive sequences may be unstable. ES1301 is Tets, which allows for cycling between antibiotic resistances. BMH 71-18 is Tetr; therefore, it can only be used to select Ampr or CATr mutants.

ES1301 mutS Genotype: lacZ53, mutS201::Tn5, thyA36, rha-5, metB1, deoC, IN(rrnD–rrnE).

BMH 71-18 mutS Genotype: thi, supE, Δ(lac-proAB), [mutS::T...

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Bacterial Strain ES1301 mutS, Glycerol Stock (noncompetent)

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Bacterial Strain ES1301 mutS, Glycerol Stock (noncompetent)

 Components

  • Bacterial Strain ES1301 mutS

    Q613A1 x 200μl
200μl
- Q6131 $ 43.00 Add to cart

Bacterial Strain BMH 71-18 mutS, Glycerol Stock (noncompetent)

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Bacterial Strain BMH 71-18 mutS, Glycerol Stock (noncompetent)

 Components

  • Bacterial Strain BMH 71-18 mutS

    Q632A1 x 500μl
500μl
- Q6321 $ 56.00 Add to cart


Storage Conditions

Store at –70°C.

For product intended use please see Patents & Disclaimers tab.

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