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Promega Corporation

ProFluor® Tyrosine Phosphatase Assay

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The ProFluor® Tyrosine Phosphatase Assay measures purified tyrosine phosphatase enzyme activity in a multiwell plate format and involves "add-mix-read" steps only—ideal for high-throughput applications. Tyrosine phosphatases tested with the assay include PTP-1B, CD45, LAR PTPase and YOP-51. The assay begins with a standard phosphatase reaction performed with a provided phosphorylated bisamide rhodamine 110 peptide substrate (PTPase R110 Substrate) and Control AMC Substrate that serves as a control for compounds that may inhibit the protease. Following the phosphatase reaction, a termination buffer containing a pro...

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Product Size Conc. Catalog # *List Price Order QTY Add to Cart

ProFluor® Tyrosine Phosphatase Assay

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ProFluor® Tyrosine Phosphatase Assay

 Components

  • Stabilizer Reagent

    V306C1 x 60μl
  • Protease Reagent

    V309A1 x 240μl
  • Control AMC Substrate

    V325A1 x 12μl
  • 5X Reaction Buffer B

    V334A1 x 6ml
  • Sodium Vanadate

    V343A1 x 200μl
  • PTPase R110 Substrate

    V345A1 x 12μl
  • 5X Termination Buffer C

    V346A1 x 5ml
4 plate
- V1280 $ 804.00 Add to cart

ProFluor® Tyrosine Phosphatase Assay

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ProFluor® Tyrosine Phosphatase Assay

 Components

  • Stabilizer Reagent

    V306D1 x 120μl
  • Protease Reagent

    V309B1 x 480μl
  • Control AMC Substrate

    V325B1 x 24μl
  • 5X Reaction Buffer B

    V334B1 x 12ml
  • Sodium Vanadate

    V343A1 x 200μl
  • PTPase R110 Substrate

    V345B1 x 24μl
  • 5X Termination Buffer C

    V346B1 x 10ml
8 plate
- V1281 $ 1,341.00 Add to cart


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Storage Conditions

Store the entire system at –20°C. Protect the PTPase R110 Substrate and Control AMC Substrate from light.

For product intended use please see Patents & Disclaimers tab.

Use Restrictions

V1280, V1281 For Research Use Only. Not for Use in Diagnostic Procedures.

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