SignaTECT® Protein Kinase Assay Systems

The SignaTECT® Protein Kinase Assay Systems contain the proprietary SAM²® Biotin Capture Membrane, which offers significant advantages over other radioactive technologies for assaying protein kinases. The streptavidin-coated SAM²® Membranes possess high binding capacity and high specificity characteristics, which produce lower backgrounds and higher signal-to-noise ratios compared to the traditional P81 phosphocellulose method of capture and measurement. The perforated and numbered membrane allows researchers to measure from 1 up to 96 kinase reactions. The SAM²® Membrane format does not require as much "hands-on"...

Expand to Read More »

The SignaTECT® Protein Kinase Assay Systems contain the proprietary SAM²® Biotin Capture Membrane, which offers significant advantages over other radioactive technologies for assaying protein kinases. The streptavidin-coated SAM²® Membranes possess high binding capacity and high specificity characteristics, which produce lower backgrounds and higher signal-to-noise ratios compared to the traditional P81 phosphocellulose method of capture and measurement. The perforated and numbered membrane allows researchers to measure from 1 up to 96 kinase reactions. The SAM²® Membrane format does not require as much "hands-on" manipulation as other methods used to measure kinase activity. Following the kinase reaction, samples are spotted onto the SAM²® Membrane, and a series of short wash steps are performed to remove nonspecific label. The process is complete in less than 1 hour. In addition, the nature of the SAM²® Membrane allows it to be used under a variety of buffer/reaction conditions (e.g., cell extracts), which many other methods do not allow. Lastly, the high binding capacity allows use of the SignaTECT® Systems for kinetic studies.

Each system contains highly specific biotinylated peptide substrates for the appropriate kinase as well as the necessary reaction components. The researcher must supply [γ-³²P]ATP.

Applications

For more information, see the Protocols & Applications Guide.

« Collapse Content