CAMK4 Kinase Enzyme System

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Recombinant full-length human CAMK4 was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag. CAMK4 is a multifunctional serine/threonine protein kinase and a member of Ca²⁺/calmodulin-dependent protein kinase family. CAMK4 is localized in neurons in the hippocampus, amygdala, anterior cingulate cortex, somatosensory cortex and insular cortex. CAMK4 is involved in neural activity-dependent signaling in the neuronal nucleus and thought to play an important role in the consolidation/retention of hippocampus-dependent long-term memory.

ADP-Glo™ Kinase Assay is ...

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See All Kinase Enzyme Systems
Recombinant full-length human CAMK4 was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag. CAMK4 is a multifunctional serine/threonine protein kinase and a member of Ca²⁺/calmodulin-dependent protein kinase family. CAMK4 is localized in neurons in the hippocampus, amygdala, anterior cingulate cortex, somatosensory cortex and insular cortex. CAMK4 is involved in neural activity-dependent signaling in the neuronal nucleus and thought to play an important role in the consolidation/retention of hippocampus-dependent long-term memory.

ADP-Glo™ Kinase Assay is a luminescent kinase assay that measures ADP formed from a kinase reaction; ADP is converted into ATP, which is a substrate in a reaction catalyzed by Ultra-Glo™ Luciferase that produces light. The luminescent signal positively correlates with ADP amount and kinase activity. The assay is well suited for measuring the effects chemical compounds have on the activity of a broad range of purified kinases, making it ideal for both primary screening as well as kinase selectivity profiling. The ADP-Glo™ Kinase Assay can be used to monitor the activity of virtually any ADP-generating enzyme (e.g., kinase or ATPase) using up to 1mM ATP.

Kinase Enzyme System contains:

• Kinase: CAMK4, 10μg (Human, recombinant full-length). MW: ~79kDa.
• Substrate: Autocamtide-2 (KKALRRQETVDAL-amide); derived from the autophosphorylation site (amino acid 283–290) on CaMKII.
• Other: Reaction Buffer, DTT, Ca²⁺/Calmodulin solution.

CAMK4 NCBI Database Entry.

Human Kinase Enzyme Systems and Applications.

Features - Benefits

• Profile More Compounds In-House: ADP-Glo™ Kinase Assay + Kinase Enzyme System is optimized so that you are up and running in no time.
• Complete Systems: The Kinase Enzyme systems include a recombinant kinase enzyme, a substrate appropriate for the enzyme, a reaction buffer, DTT and MnCl₂ to add fresh to the buffer.
• Obtain Reliable Results: The broad dynamic range, the ease of use and better sensitivity obtained with ADP-Glo™ Kinase Assay result in less ambiguous data.

Notes

Kinase Enzyme System manufactured by SignalChem.
Bulk quantities available upon request.

References

1. Sikela, J.M. et al. (1989) Genomics 4, 21–7.
2. Kang, H. et al. (2001) Cell 106, 771–83.

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