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See All Kinase Enzyme Systems
Recombinant full-length human KHS1 was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag. KHS1 belongs to the serine/threonine kinase family that has a STE20-like protein kinase domain, which stimulates the stress-activated protein kinase (SAPK, also known as Jun kinase or JNK) pathway (1). It is a mitogen-activated protein kinase kinase kinase kinase 5, termed germinal center kinase related (GCKR). Recently, the KH domain of Escherichia coli polynucleotide phosphorylase has been reported to be necessary for autoregulation and growth at low temperature (2).
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ADP-Glo™ Kinase Assay is a luminescent kinase assay that measures ADP formed from a kinase reaction; ADP is converted into ATP, which is a substrate in a reaction catalyzed by Ultra-Glo™ Luciferase that produces light. The luminescent signal positively correlates with ADP amount and kinase activity. The assay is well suited for measuring the effects chemical compounds have on the activity of a broad range of purified kinases, making it ideal for both primary screening as well as kinase selectivity profiling. The ADP-Glo™ Kinase Assay can be used to monitor the activity of virtually any ADP-generating enzyme (e.g., kinase or ATPase) using up to 1mM ATP.
Kinase Enzyme System contains:
KHS1 NCBI Database Entry.
Human Kinase Enzyme Systems and Applications.
Kinase Enzyme System manufactured by SignalChem.
Bulk quantities available upon request.
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KHS1 Kinase Enzyme System
ADP-Glo™ Kinase Assay
Upon receipt, centrifuge the kinase and dispense it into smaller quantities. Store the kinase at –70°C and the rest of the components at –20°C.
For product intended use please see Patents & Disclaimers tab.
V4108, V6441, V4109 For Research Use Only. Not for Use in Diagnostic Procedures.
V4109 U.S. Pat. Nos. 7,083,911, 7,452,663 and 7,732,128 and other patents.
V4109 U.S. Pat. No. 7,700,310 and other patents and patents pending.
V4109 U.S. Pat. Nos. 7,741,067, 8,361,739 and 8,603,767 and other patents and patents pending.
V4109 U.S. Pat. Nos. 6,602,677, 7,241,584, 8,030,017 and 8,822,170 and other patents and patents pending.
V4109 U.S. Pat. No. 8,183,007 and other patents and patents pending.
V4109 Licensed from Lonza Nottingham Ltd. under U.S. Pat. Nos. 6,599,711 and 6,911,319 and other pending and issued patents.
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