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Promega Corporation

EGF Receptor

Epidermal Growth Factor Receptor (EGF Receptor) is a cell- surface glycoprotein composed of a single polypeptide chain that binds the peptide Epidermal Growth Factor (EGF). The EGF Receptor consists of an extracellular ligand binding domain, a single transmembrane region and a cytoplasmic intrinsic tyrosine kinase domain. Upon ligand binding, the EGF Receptor autophosphorylates, activating the tyrosine kinase domain of the EGF Receptor.

EGF Receptor is immunopurified from the A431 cell line following a procedure detailed by Weber et al. The purified EGF Receptor does possess tyrosine kinase activity due to the bound E...

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EGF Receptor

- V5551 Please Enquire

Storage Buffer

20mM HEPES (pH 7.4), 1mM EDTA, 1mM DTT, 130mM NaCl, 0.05% Triton® X-100, 10% glycerol, 5μM EGF.

Storage Conditions

Store at –70°C.

Unit Definition

One unit is the amount of EGF Receptor required to catalyze the transfer of 1pmol of phosphate onto Angiotensin II per minute at 30°C.

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