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Promega Corporation

PepTag® Non-Radioactive Protein Kinase Assays

The PepTag® Non-Radioactive Protein Kinase Assay Systems provide a rapid, sensitive and non-radioactive method to detect either Protein Kinase C (PKC) or Protein Kinase A (PKA) activity. The PepTag® Assays use brightly colored, fluorescent peptide substrates that are highly specific for PKC (PepTag® C1 Peptide-PLSRTLSVAAK) and PKA (PepTag® A1 Peptide-LRRASLG). Phosphorylation of the peptide alters the net charge from +1 to –1. This change in the net charge allows the phosphorylated and nonphosphorylated versions of the substrate to be rapidly separated on an agarose gel at neutral pH. Using fluorescent detection, ...

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PepTag® Non-Radioactive PKC Assay

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PepTag® Non-Radioactive PKC Assay

 Components

  • Protein Kinase C

    V526A1 x 0.5μg
  • PepTag® C1 Peptide

    V535A1 x 650μl
  • PepTag® PKC Reaction 5X Buffer

    V536A1 x 650μl
  • Gel Solubilization Solution

    V537A1 x 10ml
  • PKC Activator 5X Solution

    V553A1 x 650μl
  • Peptide Protection Solution

    V572A1 x 250μl
120 reactions
- V5330 $ 338.00 Add to cart

PepTag® Non-Radioactive cAMP-Dependent Protein Kinase Assay

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PepTag® Non-Radioactive cAMP-Dependent Protein Kinase Assay

 Components

  • cAMP-Dependent Protein Kinase, Catalytic Subunit

    V516A1 x 2,500u
  • Gel Solubilization Solution

    V537A1 x 10ml
  • PepTag® A1 Peptide

    V538A1 x 650μl
  • PepTag® PKA Reaction 5X Buffer

    V539A1 x 650μl
  • PKA Activator 5X Solution

    V540A1 x 650μl
  • Peptide Protection Solution

    V572A1 x 250μl
120 reactions
- V5340 $ 338.00 Add to cart


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Storage Conditions

Store at –70°C.

For product intended use please see Patents & Disclaimers tab.

Use Restrictions

V5330, V5340 For Research Use Only. Not for Use in Diagnostic Procedures.

Patents - Disclaimers

V5330, V5340 Licensed for research and development purposes only under U.S. Pat. No. 5,120,644.

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