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Promega Corporation

ProFluor® Src-Family Kinase Assay

The ProFluor® Src-Family Kinase Assay measures the activity of purified Src-family tyrosine kinases (Src, Lck, Lyn, Fyn, and Hck tested) in a multiwell plate format and involves "add-mix-read" steps only—ideal for high-throughput applications. The assay begins with a standard kinase reaction performed with a provided Src-family kinase bisamide rhodamine 110 peptide substrate. Following the kinase reaction, a termination buffer containing a protease reagent is added, which simultaneously stops the kinase reaction and removes amino acids specifically from the nonphosphorylated substrate, liberating highly fluorescen...

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Product Size Conc. Catalog # *List Price QTY Add to Cart

ProFluor® Src-Family Kinase Assay

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ProFluor® Src-Family Kinase Assay

 Components

  • rATP

    P113C1 x 250μl
  • Stabilizer Reagent

    V306A1 x 12μl
  • 5X Reaction Buffer A

    V307A1 x 6ml
  • 5X Termination Buffer A

    V308A1 x 5ml
  • Protease Reagent

    V309A2 x 240μl
  • Control AMC Substrate

    V325A1 x 12μl
  • MnCl2

    V342A1 x 1ml
  • Sodium Vanadate

    V343A1 x 200μl
  • Src-Family Kinase R110 Substrate

    V344A1 x 12μl
4 plate
- V1270 $ 804.00 Add to cart


Storage Conditions

For long-term storage, store the system at –20°C. Protect the Src-Family Kinase R110 Substrate and Control AMC Substrate from light. Avoid multiple freeze-thaw cycles or exposure to frequent temperature changes. These fluctuations can greatly alter product stability.

For product intended use please see Patents & Disclaimers tab.

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