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Promega Corporation

ProFluor® PKA Assay

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The ProFluor® PKA Assay measures protein kinase A activity using purified kinase in a multiwell plate format and involves "add-mix-read" steps only—ideal for high-throughput applications. The assay begins with a standard kinase reaction performed with a provided PKA bisamide rhodamine 110 peptide substrate. Following the kinase reaction, a termination buffer containing a protease reagent is added, which simultaneously stops the kinase reaction and removes amino acids specifically from the nonphosphorylated PKA substrate, liberating highly fluorescent rhodamine 110. Phosphorylated PKA substrate, however, is resista...

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Product Size Conc. Catalog # *List Price QTY Add to Cart

ProFluor® PKA Assay

4 plate
- V1240 $ 804.00 Add to cart


Storage Conditions

Store the entire system at –20°C. Protect the PKA R110 Substrate from light. For best results, make solutions fresh and use immediately. System components should be thawed on ice and returned to –20°C as soon as possible. The PKA R110 Substrate is provided in 100% DMSO and therefore requires thawing at room temperature.

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