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See All Kinase Enzyme Systems
Recombinant full-length human MAPKAPK3 was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag. MAPKAPK3 is closely related to MAPKAPK2 sharing 72% nucleotide and 75% amino acid identity (1). MAPKAPK3 is activated by growth inducers and stress stimulation of cells. In vitro studies demonstrated that ERK, p38 MAP kinase and Jun N-terminal kinase were all able to phosphorylate and activate this kinase, which suggested the role of MAPKAPK3 as an integrative element of signaling in both mitogen and stress responses (2).
ADP-Glo™ Kinase Assay is a luminescent kina...
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ADP-Glo™ Kinase Assay is a luminescent kinase assay that measures ADP formed from a kinase reaction; ADP is converted into ATP, which is a substrate in a reaction catalyzed by Ultra-Glo™ Luciferase that produces light. The luminescent signal positively correlates with ADP amount and kinase activity. The assay is well suited for measuring the effects chemical compounds have on the activity of a broad range of purified kinases, making it ideal for both primary screening as well as kinase selectivity profiling. The ADP-Glo™ Kinase Assay can be used to monitor the activity of virtually any ADP-generating enzyme (e.g., kinase or ATPase) using up to 1mM ATP.
Kinase Enzyme System contains:
MAPKAPK3 NCBI Database Entry.
Human Kinase Enzyme Systems and Applications.
Kinase Enzyme System manufactured by SignalChem.
Bulk quantities available upon request.
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MAPKAPK3 Kinase Enzyme System
ADP-Glo™ Kinase Assay
Upon receipt, centrifuge the kinase and dispense it into smaller quantities. Store the kinase at –70°C and the rest of the components at –20°C.
For product intended use please see Patents & Disclaimers tab.
V4026, V6444, V4027 For Research Use Only. Not for Use in Diagnostic Procedures.
V4027 U.S. Pat. Nos. 7,083,911, 7,452,663 and 7,732,128 and other patents.
V4027 U.S. Pat. No. 7,700,310 and other patents and patents pending.
V4027 U.S. Pat. Nos. 7,741,067, 8,361,739, 8,603,767 and other patents and patents pending.
V4027 U.S. Pat. Nos. 6,602,677, 7,241,584, 8,030,017 and 8,822,170 and other patents and patents pending.
V4027 U.S. Pat. No. 8,183,007 and other patents and patents pending.
V4027 Licensed from Lonza Nottingham Ltd. under U.S. Pat. Nos. 6,599,711 and 6,911,319 and other pending and issued patents.
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