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See All Kinase Enzyme Systems
Recombinant full-length human AMPK (combination of A2/B1/G1 subunits) was expressed by baculovirus in Sf9 insect cells using a C-terminal His tag. AMPK (A2/B1/G1) plays a key role in insulin signaling pathway and is a major therapeutic target for the treatment of diabetes (1). AMPK is viewed as a fuel sensor for glucose and lipid metabolism by modulating the activity of the autonomous nervous system in vivo. Short-term overexpression of a constitutively active form of AMPK in the liver leads to mild hypoglycemia and fatty liver due to increased fatty acid utilization (2).
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ADP-Glo™ Kinase Assay is a luminescent kinase assay that measures ADP formed from a kinase reaction; ADP is converted into ATP, which is a substrate in a reaction catalyzed by Ultra-Glo™ Luciferase that produces light. The luminescent signal positively correlates with ADP amount and kinase activity. The assay is well suited for measuring the effects chemical compounds have on the activity of a broad range of purified kinases, making it ideal for both primary screening as well as kinase selectivity profiling. The ADP-Glo™ Kinase Assay can be used to monitor the activity of virtually any ADP-generating enzyme (e.g., kinase or ATPase) using up to 1mM ATP.
Kinase Enzyme System contains:
AMPK (A2/B1/G1) NCBI Database Entry.
Human Kinase Enzyme Systems and Applications.
Kinase Enzyme System manufactured by SignalChem.
Bulk quantities available upon request.
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AMPK (A2/B1/G1) Kinase Enzyme System
ADP-Glo™ Kinase Assay
Upon receipt, centrifuge the kinase and dispense it into smaller quantities. Store the kinase at –70°C and the rest of the components at –20°C.
For product intended use please see Patents & Disclaimers tab.
V4014, V6275, V4015 For Research Use Only. Not for Use in Diagnostic Procedures.
V4015 U.S. Pat. Nos. 7,083,911, 7,452,663 and 7,732,128 and other patents.
V4015 U.S. Pat. No. 7,700,310 and other patents and patents pending.
V4015 U.S. Pat. Nos. 7,741,067, 8,361,739, 8,603,767 and other patents and patents pending.
V4015 U.S. Pat. Nos. 6,602,677, 7,241,584 and 8,030,017 and other patents and patents pending.
V4015 U.S. Pat. No. 8,183,007 and other patents and patents pending.
V4015 Licensed from Lonza Nottingham Ltd. under U.S. Pat. Nos. 6,599,711 and 6,911,319 and other pending and issued patents.
V4015 The method of recombinant expression of Coleoptera luciferase is covered by U.S. Pat. Nos. 5,583,024, 5,674,713 and 5,700,673.
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