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Promega Corporation

DNA-PK Kinase Enzyme System

See All Kinase Enzyme Systems
Native human DNA-Dependent Protein Kinase (DNA-PK) purified from HeLa cells. DNA-PK consists of an approximately 460kDa catalytic subunit and a heterodimeric DNA-binding subunit (Ku) containing an 85kDa and an 70kDa peptide (1). The gene sequence can be found at accession number NM_006904.

ADP-Glo™ Kinase Assay is a luminescent kinase assay that measures ADP formed from a kinase reaction; ADP is converted into ATP, which is a substrate in a reaction catalyzed by Ultra-Glo™ Luciferase that produces light. The luminescent signal positively correlates with ADP amount and kinase...

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DNA-PK Kinase Enzyme System

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DNA-PK Kinase Enzyme System

 Components

  • DTT

    P117A1 x 100μl
  • 5X Reaction Buffer A

    V307C1 x 1.5ml
  • DNA-Dependent Protein Kinase Substrate

    V567A1 x 1mg
  • DNA-Dependent Protein Kinase

    V581A1 x 2,500u
  • DNA-PK Activation Buffer

    V791A2 x 300μl
2,500 units
- V4106 $ 406.00 Add to cart

ADP-Glo™ Kinase Assay + DNA-PK Kinase Enzyme System

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ADP-Glo™ Kinase Assay + DNA-PK Kinase Enzyme System

 Components

  • DNA-PK Kinase Enzyme System

    V41061 x 2,500 units
  • ADP-Glo™ Kinase Assay

    V91011 x 1,000 assays
1 each
- V4107 $ 808.00 Add to cart


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Storage Buffer

25mM HEPES (pH 7.5), 50mM KCl, 0.2mM EDTA, 10mM MgCl2, 1mM DTT, 10% glycerol.

DNA-PK Activation Buffer

100µg/ml calf thymus DNA in 1X TE.

Storage Conditions

Upon receipt, centrifuge the kinase and dispense it into smaller quantities. Store the kinase at –70°C and the rest of the components at –20°C.

Unit Definition

One unit is the amount of enzyme required to incorporate 1pmol of phosphate into DNA-PK Peptide Substrate (Cat.# V5671) in 1 minute at 30°C.

For product intended use please see Patents & Disclaimers tab.

Use Restrictions

V4106, V4107 For Research Use Only. Not for Use in Diagnostic Procedures.

Patents - Disclaimers

V4107 U.S. Pat. Nos. 7,083,911, 7,452,663 and 7,732,128 and other patents.

V4107 U.S. Pat. No. 7,700,310 and other patents and patents pending.

V4107 U.S. Pat. No. 7,741,067 and other patents and patents pending.

V4107 U.S. Pat. Nos. 6,602,677, 7,241,584 and 8,030,017 and other patents and patents pending.

V4107 U.S. Pat. No. 8,183,007 and other patents pending.

V4107 Licensed from Lonza Nottingham Ltd. under U.S. Pat. Nos. 6,599,711 and 6,911,319 and other pending and issued patents.

V4107 The method of recombinant expression of Coleoptera luciferase is covered by U.S. Pat. Nos. 5,583,024, 5,674,713 and 5,700,673.

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