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Promega Corporation

PKC Kinase Enzyme System

See All Kinase Enzyme Systems
Native rat brain Protein Kinase C (PKC) is purified according to the method of Walton et al. (1). The purified PKC consists primarily of α, β and γ isoforms with lesser amounts of δ and ζ isoforms. The gene accession number for PKCα is NM_001105713.1.

ADP-Glo™ Kinase Assay is a luminescent kinase assay that measures ADP formed from a kinase reaction; ADP is converted into ATP, which is a substrate in a reaction catalyzed by Ultra-Glo™ Luciferase that produces light. The luminescent signal positively correlates with ADP amount and kinase acti...

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PKC Kinase Enzyme System

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PKC Kinase Enzyme System

 Components

  • PKC Activation 5X Buffer

    V226A4 x 200μl
  • Protein Kinase C

    V526A1 x 0.5μg
  • Neurogranin(28–43) PKC Peptide Substrate

    V561A1 x 1mg
  • PKC Coactivation 5X Buffer

    V592A2 x 600μl
0.5μg
- V4504 $ 406.00 Add to cart

ADP-Glo™ Kinase Assay + PKC Kinase Enzyme System

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ADP-Glo™ Kinase Assay + PKC Kinase Enzyme System

 Components

  • PKC Kinase Enzyme System

    V45041 x 0.5μg
  • ADP-Glo™ Kinase Assay

    V91011 x 1,000 assays
1 each
- V4505 $ 808.00 Add to cart


Storage Buffer

20mM Tris-HCl (pH 7.5), 2mM EGTA, 2mM EDTA, 1mM DTT, 10mM potassium phosphate (pH 7.5), 50% glycerol and 0.05% Triton® X-100.

PKC Activation 5X Buffer

1.6mg/ml phosphatidylserine, 0.16mg/ml diacylglycerol, 100mM Tris-HCl (pH 7.5), 50mM MgCl2.

PKC Coactivation 5X Buffer

1.25mM EGTA, 2mM CaCl2, 0.5mg/ml BSA.

Storage Conditions

Upon receipt, centrifuge the kinase and dispense it into smaller quantities. Store the kinase at –70°C and the rest of the components at –20°C.

For product intended use please see Patents & Disclaimers tab.

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