350mM potassium phosphate (pH 6.8), 0.1mM DTT.
Upon receipt, centrifuge the kinase and dispense it into smaller quantities. Store the kinase at –70°C and the rest of the components at –20°C.
One unit is the amount of enzyme required to incorporate 1pmol of phosphate into casein in 1 minute. The assay buffer is 40mM Tris-HCl (pH 7.4), 20mM magnesium acetate, 0.2mM ATP and 30,000cpm/μl [γ-[32P] ATP].
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