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Promega Corporation

ADP-Glo™ Max Assay

The ADP-Glo™ Max Assay is a luminescent ADP detection assay that provides a universal, homogeneous, high-throughput screening method to measure ATPase or kinase activity by quantifying the amount of ADP produced in a reaction. The assay can be used to monitor the activity of virtually any ADP-generating enzyme (e.g., kinase or ATPase) when higher ATP concentration is required (up to 5mM). The ADP-Glo™ Max Assay produces a strong signal that positively correlates with enzyme activity and can be adapted to a multitude of plate formats.

The assay is performed in two steps: first, after the completion of the ADP...

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ADP-Glo™ Max Assay

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ADP-Glo™ Max Assay

 Components

  • ADP-Glo™ Max Detection Buffer

    V701A1 x 10ml
  • ADP-Glo™ Max Detection Substrate

    V702A1 x 2ml
  • Ultra Pure ATP, 100mM

    V703A1 x 250μl
  • ADP-Glo™ Reagent

    V912A1 x 5ml
  • ADP, 10mM

    V916A2 x 500μl
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  • This product can be added to a

  • Helix Freezer

This product is available through the Promega Helix onsite stocking program in a –20°C Helix Freezer. The program offers numerous convenient solutions to meet your lab's needs. Helix Freezers are available in two sizes: 5.7 cubic ft. or 9.7 cubic ft.


Click here to learn more about the Helix Collection »


Already a Helix Customer?

Request to add this product to your Helix »


1,000 assays
- V7001 $ 580.00 Add to cart

ADP-Glo™ Max Assay

Close Window

ADP-Glo™ Max Assay

 Components

  • ADP-Glo™ Max Detection Buffer

    V701B1 x 100ml
  • ADP-Glo™ Max Detection Substrate

    V702B1 x 20ml
  • Ultra Pure ATP, 100mM

    V703B1 x 2.5ml
  • ADP-Glo™ Reagent

    V912B1 x 50ml
  • ADP, 10mM

    V916B1 x 5ml
Close Window
  • This product can be added to a

  • Helix Freezer

This product is available through the Promega Helix onsite stocking program in a –20°C Helix Freezer. The program offers numerous convenient solutions to meet your lab's needs. Helix Freezers are available in two sizes: 5.7 cubic ft. or 9.7 cubic ft.


Click here to learn more about the Helix Collection »


Already a Helix Customer?

Request to add this product to your Helix »


10,000 assays
- V7002 $ 3,344.00 Add to cart


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Storage Conditions

Store the system at –20°C. Before use, thaw all components completely at room temperature. Once thawed, mix all components thoroughly before use. Because ATP is naturally prone to hydrolysis after freeze-thaw cycles dispense into single-use aliquots and store at –20°C. Once prepared, dispense, ADP-Glo™ Max Detection Reagent (ADP-Glo™ Max Detection Buffer + Substrate) into aliquots and store at –20°C. ADP-Glo™ Max Detection Buffer may form a precipitate when thawed. See Section 3.A of the Technical Manual for a protocol to dissolve any precipitate. For convenience, ADP-Glo™ Reagent and ADP-Glo™ Max Detection Reagent may be kept at room temperature (22°C) for 24 hours without loss of signal.

For product intended use please see Patents & Disclaimers tab.

8051MBFigure 1. Principle of the ADP-Glo™ Max Assay.

The assay is performed in two steps: 1) after the ATPase or kinase reaction, ADP-Glo™ Reagent is added to terminate the reaction and deplete the remaining ATP; and 2) the ADP-Glo™ Max Detection Reagent is added to convert ADP to ATP and allow the newly synthesized ATP to be measured using a luciferase/luciferin reaction. The light generated correlates to ADP present and ATPase activity.

Use Restrictions

V7001, V7002 For Research Use Only. Not for Use in Diagnostic Procedures.

Patents - Disclaimers

V7001, V7002 U.S. Pat. Nos. 7,083,911, 7,452,663 and 7,732,128 and other patents.

V7001, V7002 U.S. Pat. No. 7,700,310 and other patents and patents pending.

V7001, V7002 U.S. Pat. No. 7,741,067 and other patents and patents pending.

V7001, V7002 U.S. Pat. Nos. 6,602,677, 7,241,584 and 8,030,017 and other patents and patents pending.

V7001, V7002 U.S. Pat. No. 8,183,007 and other patents pending.

V7001, V7002 Licensed from Lonza Nottingham Ltd. under U.S. Pat. Nos. 6,599,711 and 6,911,319 and other pending and issued patents.

V7001, V7002 The method of recombinant expression of Coleoptera luciferase is covered by U.S. Pat. Nos. 5,583,024, 5,674,713 and 5,700,673.

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