CellTiter 96® AQ_ueous Non-Radioactive Cell Proliferation Assay (MTS)

The CellTiter 96® AQ_ueous Non-Radioactive Cell Proliferation Assay is a homogeneous, colorimetric method for determining the number of viable cells in proliferation, cytotoxicity or chemosensitivity assays. The CellTiter 96® AQ_ueous Assay is composed of solutions of a novel tetrazolium compound [3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium, inner salt; MTS] and an electron coupling reagent (phenazine methosulfate) PMS. MTS is bioreduced by cells into a formazan product that is soluble in tissue culture medium. The absorbance of the formazan product at 490nm can be measur...

Expand to Read More »

The CellTiter 96® AQ_ueous Non-Radioactive Cell Proliferation Assay is a homogeneous, colorimetric method for determining the number of viable cells in proliferation, cytotoxicity or chemosensitivity assays. The CellTiter 96® AQ_ueous Assay is composed of solutions of a novel tetrazolium compound [3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium, inner salt; MTS] and an electron coupling reagent (phenazine methosulfate) PMS. MTS is bioreduced by cells into a formazan product that is soluble in tissue culture medium. The absorbance of the formazan product at 490nm can be measured directly from 96-well assay plates without additional processing. The conversion of MTS into the aqueous soluble formazan product is accomplished by dehydrogenase enzymes found in metabolically active cells. The quantity of formazan product as measured by the amount of 490nm absorbance is directly proportional to the number of living cells in culture.

If you currently use a [³H]-thymidine incorporation assay, addition of the combined MTS/PMS solution can be substituted for [³H]-thymidine at the time point in the assay when the pulse of radioactive thymidine is usually added. Data from proliferation bioassays comparing the CellTiter 96® AQ_ueous Assay and [³H]-thymidine incorporation show similar results. This is in agreement with similar radioactivity incorporation studies performed using the original CellTiter 96® Assay.

CellTiter 96® AQ_ueous MTS Reagent Powder is a novel tetrazolium compound for use in colorimetric assays for determining the number of viable cells in proliferation, cytotoxicity or chemosensitivity assays. It is provided in powdered form.

Features - Benefits

• Easy to Use: Combine provided MTS and PMS solutions, add to cells, incubate and read absorbance.
• Fast: Perform the assay in a 96-well plate with no washing or cell harvesting. Also eliminates solubilization steps because the MTS formazan product is soluble in tissue culture medium.
• Non-Radioactive: Requires no scintillation cocktail or radioactive waste disposal (unlike [³H]-thymidine).
• Flexible: Plates can be read and returned to incubator for further color development (unlike MTT).
• Safe: Requires no volatile organic solvent to solubilize the formazan product (unlike MTT).

Applications

• Cell proliferation.
• Cytotoxicity.
• Cell viability.
• Chemosensitivity.
• Cell attachment determinations.
• Chemotaxis.

For more information, see the Protocols & Applications Guide.

References

1. Barltrop, J.A. et al. (1991) Bioorg. & Med. Chem. Lett. 1, 611.
2. Cory, A.H. et al. (1991) Cancer Commun. 3, 207–12.
3. Riss, T.L. and Moravec, R.A. (1993) Mol. Biol. Cell 3 (Suppl.), 184a.
4. CellTiter 96® Non-Radioactive Cell Proliferation Assay Technical Bulletin, #TB112, Promega Corporation.

« Collapse Content