Caspase-Glo® 2 Assay Systems

The Caspase-Glo® 2 Assay is a homogeneous, luminescent assay that measures caspase-2 activity. Caspase-2 is a member of the cysteine aspartic acid-specific protease family. The Caspase-Glo® 2 Assay provides a luminogenic substrate (Z-VDVAD-aminoluciferin) in a reagent optimized for caspase-2 and luciferase activity. A single reagent is added to test samples, resulting in caspase cleavage of the substrate and generation of a glow-type luminescent signal produced by luciferase. Luminescence is proportional to the amount of caspase activity present. The assay system may be used with purified enzyme preparations and i...

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The Caspase-Glo® 2 Assay is a homogeneous, luminescent assay that measures caspase-2 activity. Caspase-2 is a member of the cysteine aspartic acid-specific protease family. The Caspase-Glo® 2 Assay provides a luminogenic substrate (Z-VDVAD-aminoluciferin) in a reagent optimized for caspase-2 and luciferase activity. A single reagent is added to test samples, resulting in caspase cleavage of the substrate and generation of a glow-type luminescent signal produced by luciferase. Luminescence is proportional to the amount of caspase activity present. The assay system may be used with purified enzyme preparations and is ideal for automated high-throughput screening of inhibitors.

Features - Benefits

• Broad Dynamic Range: The assay is linear over four logs of caspase-2 concentration and can detect caspase-2 activity at concentrations as low as 0.2mU/ml.
• High-Quality Assay: The assay demonstrates an excellent Z´-factor value of 0.85 in 384-well plates using 0.05U/ml of enzyme.
• Increased Accuracy: The superior sensitivity over fluorescence-based caspase assays allows inhibitor studies to be performed below the K_m.
• Batch Processing Capability: The coupled-enzyme, homogeneous format results in a continuous signal, providing excellent stability and allowing plates to be read over an extended period of time. Luminometers with reagent injectors are not required.

Applications

• Caspase-2 inhibitor studies.

Notes

Cat.# G0940 provides sufficient reagents for 100 assays at 100μl/assay or 200 assays at 50μl/assay in 96-well plates or 400 assays at 25μl/assay in 384-well plates. Cat.# G0941 provides sufficient reagents for 500 assays at 100μl/assay or 1,000 assays at 50μl/assay in 96-well plates or 2,000 assays at 25μl/assay in 384-well plates.

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