This country code will remain if no action is taken to change it.
Our website uses functional cookies that do not collect any personal information or track your browsing activity. When you select your country, you agree that we can place these functional cookies on your device.
The CellTiter 96® Assay is a collection of qualified reagents that provide a convenient method of determining viable cell number. The CellTiter 96® Assay is a modification of the MTT assay method described by Mosmann and incorporates several improvements to the method that address previous technical problems including: 1) serum protein precipitation caused by adding organic solvent; 2) interference by phenol red; 3) incomplete solubilization of the formazan crystals resulting in lower sensitivity; and 4) stability of the colored product.
The CellTiter 96® Assay is performed by adding a premixed, optimized Dye S...
Expand to Read More »
The CellTiter 96® Assay is performed by adding a premixed, optimized Dye Solution to culture wells of a 96-well plate, usually containing various concentrations of growth factor or test substance. During a 4-hour incubation, living cells convert the MTT tetrazolium component of the Dye Solution into a formazan product. If you currently use a [3H]-thymidine incorporation assay, the addition of Dye Solution can be substituted for the pulse of radioactive thymidine at the time point in the assay when the pulse of [3H]-thymidine is usually added. The Solubilization/Stop Solution is then added to the culture wells to solubilize the formazan product, and the absorbance at 570nm is recorded using a 96-well plate reader. In addition, direct comparison between [3H]-thymidine incorporation and tetrazolium conversion have demonstrated less than a 5% difference between the two assays for determination of growth factor content of several samples.
For more information, see the Protocols & Applications Guide.
« Collapse Content
CellTiter 96® Non-Radioactive Cell Proliferation Assay
Solubilization Solution/Stop Mix
Store Dye Solution at –20°C and Solubilization/Stop Solution at room temperature.
For product intended use please see Patents & Disclaimers tab.
G4000, G4100 For Research Use Only. Not for Use in Diagnostic Procedures.
View All Reviews »
Review This Product »
We offer a range of services to help you succeed using Promega technologies. From product training to set up of automated systems and development of custom applications—our scientific support goes beyond the basics.
Ask us! We are here to help you.