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The UGT-Glo™ Assay provides a luminescent method for measuring UDP glucuronosyltransferase (UGT) activity. The UGT-Glo™ Assay is designed to measure UGT activity from a variety of sources, such as microsomes containing recombinantly expressed enzymes or microsomal preparations derived from mammalian tissues, and to test the effects of various chemicals on UGT activity.
The assay involves incubating UGT with a proluciferin substrate; a portion of the substrate gets conjugated with UDP, while the remainder is unmodified. Upon the addition of d-Cysteine, the unconjugated proluciferin is converted into luciferin an...
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The assay involves incubating UGT with a proluciferin substrate; a portion of the substrate gets conjugated with UDP, while the remainder is unmodified. Upon the addition of d-Cysteine, the unconjugated proluciferin is converted into luciferin and, in a coupled reaction with luciferase/luciferin, is converted into light. Conjugated proluciferin remains intact and does not contribute to the luminescence. Thus, the signal generated is inversely correlated with UGT activity present in the sample.
The UGT-Glo™ Assay contains two proluciferin substrates: the UGT Multienzyme Substrate, which is compatible with a wide range of UGTs, and the UGT1A4 Substrate, which reacts specifically with UGT1A4. The kit also contains Luciferin Detection Reagent and Reconstitution Buffer, UGT Buffer, d-Cysteine and UDPGA. The UGT-Glo™ Screening Systems contain the above reagents as well as the respective UGT isoforms and control membranes.
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UGT Buffer, 5X
UGT Multienzyme Substrate
Luciferin Detection Reagent
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This product is available through the Promega Helix onsite stocking program in a –20°C Helix Freezer. The program offers numerous convenient solutions to meet your lab's needs. Helix Freezers are available in two sizes: 5.7 cubic ft. or 9.7 cubic ft.
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UGT-Glo™ UGT1A1 Screening System
UGT-Glo™ UGT2B7 Screening System
Store UGT enzymes and Control Membranes at –70°C. Store remaining components at –20°C.
For product intended use please see Patents & Disclaimers tab.
UGT enzymes attach a glucuronic acid moiety to the proluciferin substrate. During the detection step, the unconjugated proluciferin is converted to luciferin by cyclization with D-cysteine and then converted into light by luciferase. Glucuronidated proluciferin does not get converted into luciferin, and thus does not produce light. Light output is inversely proportional to UGT enzymatic activity.
V2081, V2082, V2120, V2121, V2130, V2131 For Research Use Only. Not for Use in Diagnostic Procedures.
V2081, V2082, V2120, V2121, V2130, V2131 U.S. Pat. Nos. 6,602,677, 7,241,584 and 8,030,017 and other patents and patents pending.
V2081, V2082, V2120, V2121, V2130, V2131 The method of recombinant expression of Coleoptera luciferase is covered by U.S. Pat. Nos. 5,583,024, 5,674,713 and 5,700,673.
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