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Promega Corporation

Pgp-Glo™ Assay Systems

The Pgp-Glo™ Assay Systems provide the necessary reagents for performing luminescent P-glycoprotein (Pgp) ATPase assays. Pgp, also known as MDR1 and ABCB1, is a 170kDa integral plasma membrane protein that functions as an ATP-dependent drug efflux pump and plays an important role in multidrug resistance and certain adverse drug-drug interactions. Compounds that interact with Pgp can be identified as stimulators or inhibitors of its ATPase activity. Compounds that are substrates for transport by Pgp typically stimulate its ATPase activity.

The Pgp-Glo™ Assay detects the effects of compounds on recombinant hu...

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Pgp-Glo™ Assay System

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Pgp-Glo™ Assay System

 Components

  • Pgp-Glo™ Assay Buffer

    V361A1 x 10ml
  • ATP Detection Buffer

    V362A1 x 10ml
  • ATP Detection Substrate

    V363A1 x 1 vial
  • Verapamil

    V365A1 x 100μl
  • MgATP

    V366A1 x 1ml
  • Sodium Vanadate

    V367A1 x 250μl
10ml
- V3591 $ 264.00 Add to cart

Pgp-Glo™ Assay System with P-glycoprotein

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Pgp-Glo™ Assay System with P-glycoprotein

 Components

  • Pgp-Glo™ Assay Buffer

    V361A1 x 10ml
  • ATP Detection Buffer

    V362A1 x 10ml
  • ATP Detection Substrate

    V363A1 x 1 vial
  • Human Pgp Membranes, Recombinant

    V364A1 x 0.5ml
  • Verapamil

    V365A1 x 100μl
  • MgATP

    V366A1 x 1ml
  • Sodium Vanadate

    V367A1 x 250μl
10ml
- V3601 $ 529.00 Add to cart


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Storage Conditions

Store Recombinant Human Pgp Membranes at –70°C. All other components can be stored at –70°C or –20°C, protected from light.

For product intended use please see Patents & Disclaimers tab.

Stimulation of Pgp ATPase activity by verapamil. NT=not treated. TC=test compound.Figure 1. Pgp-Glo™ Assay System.

Stimulation of Pgp ATPase activity by verapamil. NT=not treated. TC=test compound.

Stimulation of Pgp ATPase activity by verapamil. NT=not treated. TC=test compound.

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Use Restrictions

V3591, V3601 For Research Use Only. Not for Use in Diagnostic Procedures.

Patents - Disclaimers

V3591, V3601 Patent Pending.

V3591, V3601 The method of recombinant expression of Coleoptera luciferase is covered by U.S. Pat. Nos. 5,583,024, 5,674,713 and 5,700,673.

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