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The pro-luciferin substrates can be used to monitor the activity of specific isoforms of cytochrome P450 or NAT2 as indicated in the name of the substrate. The Luciferin-MultiCYP is a promiscuous substrate that reacts with at least 21 P450 isoforms and is useful for measuring net CYP activity in a mixed population of P450s. The Luciferin-NAT2 is an excellent substrate for N-acetyltransferase 2 (NAT2), a phase II biotransformation enzyme that acetylates aromatic amine groups on xenobiotic compounds. This substrate shows little to no cross-reactivity with NAT1.
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Luciferin Detection Reagent must be used in combination with the pro-luciferin substrates on this page. Use V8921 with P1721, P1741, P1621, P1651 and P1661. V8931 must be used with P1671 and P1731, as these substrates are ester derivatives.
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Luciferin Detection Reagent
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This product is available through the Promega Helix onsite stocking program in a –20°C Helix Freezer. The program offers numerous convenient solutions to meet your lab's needs. Helix Freezers are available in two sizes: 5.7 cubic ft. or 9.7 cubic ft.
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Luciferin Detection Reagent with esterase
Reconstitution Buffer with esterase
For product intended use please see Patents & Disclaimers tab.
V8921, V8920, V8931, V8930, P1721, P1741, P1621, P1651, P1661, P1671, P1731 For Research Use Only. Not for Use in Diagnostic Procedures.
V8921, V8920, V8931, V8930 U.S. Pat. Nos. 6,602,677, 7,241,584 and 8,030,017 and other patents and patents pending.
V8921, V8920, V8931, V8930 The method of recombinant expression of Coleoptera luciferase is covered by U.S. Pat. Nos. 5,583,024, 5,674,713 and 5,700,673.
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