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Promega Corporation

ADCC Reporter Bioassay, Core Kits

Antibody-dependent cell-mediated cytotoxicity (ADCC) is a mechanism of action (MOA) of antibodies through which virus-infected or other diseased cells are targeted for destruction by components of the cell-mediated immune system. The ADCC Reporter Bioassay is a bioluminescent assay for quantifying Fc effector function of monoclonal antibodies as measured by activation of NFAT signaling pathway (Figure 1). The assay includes effector cells provided in frozen, thaw-and-use format and reagents and an optimized protocol to provide a bioassay that has low variability and high accuracy. Moreover the bioassay can b...

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ADCC Reporter Bioassay, Core Kit

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ADCC Reporter Bioassay, Core Kit

 Components

  • ADCC Bioassay Effector Cells

    G70111 x 1 vial
  • RPMI 1640 Medium

    G708A1 x 36ml
  • Low IgG Serum

    G711A1 x 4ml
  • Bio-Glo™ Luciferase Assay Buffer

    G719A1 x 10ml
  • Bio-Glo™ Luciferase Assay Substrate

    G720A1 x 500μl
1 each
- G7010 $ 716.00 Add to cart

ADCC Reporter Bioassay, Core Kit 5X

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ADCC Reporter Bioassay, Core Kit 5X

 Components

  • ADCC Bioassay Effector Cells 5X

    G70171 x 5 vials
  • RPMI 1640 Medium

    G708A5 x 36ml
  • Low IgG Serum

    G711A5 x 4ml
  • Bio-Glo™ Luciferase Assay Buffer

    G719A5 x 10ml
  • Bio-Glo™ Luciferase Assay Substrate

    G720A5 x 500μl
1 each
- G7018 $ 3,214.00 Add to cart


Storage Conditions

Note: The ADCC Reporter Bioassay components are shipped separately because of temperature requirements. The ADCC Bioassay Effector Cells are shipped on dry ice. The Bio-Glo™ Luciferase Assay System and Low IgG Serum are shipped on dry ice, separately from the cells. The RPMI 1640 Medium is shipped at ambient temperature.

Upon arrival, immediately transfer the vials of ADCC Bioassay Effector Cells for long-term storage below –140°C (freezer or liquid nitrogen vapor phase). The cells are sensitive, and care should be taken when handling. For safety reasons do not store cell vials submerged in liquid nitrogen. Low IgG Serum should be stored at –20°C. Avoid multiple freeze-thaw cycles. Bio-Glo™ Luciferase Assay Buffer and Bio-Glo™ Luciferase Assay Substrate should be stored at –20°C. For optimal performance, reconstituted Bio-Glo™ Luciferase Assay Reagent should be used the day of preparation. However, once reconstituted, Bio-Glo™ Luciferase Assay Reagent can be stored at –20°C for up to 6 weeks. RPMI 1640 Medium should be stored at 4°C protected from fluorescent light.

For product intended use please see Patents & Disclaimers tab.

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