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Promega Corporation

ADCC Reporter Bioassay, Complete Kit (WIL2-S)

The ADCC Reporter Bioassay, Complete Kit (WIL2-S), is suitable as a starter kit for trying the ADCC Reporter Bioassay or when the antibody of interest recognizes antigens on WIL2-S cells.

Antibody-dependent cell-mediated cytotoxicity (ADCC) is a mechanism of action (MOA) of antibodies through which virus-infected or other diseased cells are targeted for destruction by components of the cell-mediated immune system. The ADCC Reporter Bioassay is a bioluminescent assay for quantifying Fc effector function of monoclonal antibodies as measured by activation of NFAT signaling pathway (Figure 1). The assay includes effector ...

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ADCC Reporter Bioassay, Complete (WIL2-S)

1 each
- G7014 $ 857.00 Add to cart

Storage Conditions

Note: The ADCC Reporter Bioassay components are shipped separately because of temperature requirements. The ADCC Bioassay Effector Cells and Target Cells are shipped on dry ice. The Bio-Glo™ Luciferase Assay System and Low IgG Serum are shipped on dry ice, separately from the cells. The RPMI 1640 Medium is shipped at ambient temperature. The Control Ab, Anti-CD20, is shipped on gel ice.

Upon arrival, immediately transfer the vials of ADCC Bioassay Effector Cells and Target Cells for long-term storage below –140°C (freezer or liquid nitrogen vapor phase). The cells are sensitive, and care should be taken when handling. For safety reasons do not store cell vials submerged in liquid nitrogen. Low IgG Serum should be stored at –20°C. Avoid multiple freeze-thaw cycles. Store the Control Ab, Anti-CD20, at 4°C. Bio-Glo™ Luciferase Assay Buffer and Bio-Glo™ Luciferase Assay Substrate should be stored at –20°C. For optimal performance, reconstituted Bio-Glo™ Luciferase Assay Reagent should be used the day of preparation. However, once reconstituted, Bio-Glo™ Luciferase Assay Reagent can be stored at –20°C for up to 6 weeks. RPMI 1640 Medium should be stored at 4°C protected from fluorescent light.

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