TMB Stabilized Substrate for Horseradish Peroxidase

TMB Stabilized Substrate is a stable, ready-to-use TMB (3,3´, 5,5´-tetramethylbenzidine) color development substrate for localization of horseradish peroxidase-conjugated antibodies on dot blots and Western blots. It is easier to use than 4-chloro-1-naphthol (CN), which must be prepared immediately before use. TMB Stabilized Substrate comes premixed and fully diluted in a proprietary buffer containing less than 0.5% organic solvent.

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TMB Stabilized Substrate is a stable, ready-to-use TMB (3,3´, 5,5´-tetramethylbenzidine) color development substrate for localization of horseradish peroxidase-conjugated antibodies on dot blots and Western blots. It is easier to use than 4-chloro-1-naphthol (CN), which must be prepared immediately before use. TMB Stabilized Substrate comes premixed and fully diluted in a proprietary buffer containing less than 0.5% organic solvent.

Features - Benefits

• Convenient: Premixed, ready-to-use; in proprietary buffer containing less than 0.5% organic solvents.
• Stable: Stable at room temperature for 12 months.
• Sensitive: At least threefold more sensitive than 4-chloro-1-naphthol (CN); as little as 412pg of β-galactosidase detected on TMB blot as compared to 1.12ng on CN blot when detected with a β-galactosidase-specific antibody and HRP-conjugated secondary antibody.
• Long-Lasting Color: Color is much more stable than 4-chloro-1-naphthol and photographs more easily.

Applications

• Western analysis.

Note: Not recommended for use with ELISAs.

References

1. Larson, G. (1992) Promega Notes 39, 17–18.

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