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TMB Stabilized Substrate is a stable, ready-to-use TMB (3,3´, 5,5´-tetramethylbenzidine) color development substrate for localization of horseradish peroxidase-conjugated antibodies on dot blots and Western blots. It is easier to use than 4-chloro-1-naphthol (CN), which must be prepared immediately before use. TMB Stabilized Substrate comes premixed and fully diluted in a proprietary buffer containing less than 0.5% organic solvent.
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Note: Not recommended for use with ELISAs.
TMB Stabilized Substrate for Horseradish Peroxidase
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This product is available through the Promega Helix onsite stocking program in a room temperature cabinet. The program offers numerous convenient solutions to meet your lab's needs. The Helix Cabinet is available in two sizes: 7.9 cubic ft. or 25 cubic ft.
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Store at 22–25°C.
For product intended use please see Patents & Disclaimers tab.
The blot was performed on a serial dilution of lysate; each lane contains an indicated amount of total protein (µg). Primary antibody (monoclonal) used at 1:5,000; Anti-Mouse IgG, HRP Conjugate secondary antibody (Cat.# W4021) used at 1:2,500; blot imaged with TMB Stabilized Substrate for Horseradish Peroxidase (Cat.# W4121).
W4121 For Research Use Only. Not for Use in Diagnostic Procedures.
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