Press Releases
New Promega GoTaq® qPCR Master Mix Excels in Sensitivity and Stability
MADISON, WISCONSIN (September 3, 2009)
Single copy detection with GoTaq qPCR Master Mix: The GAPDH gene was amplified and detected from 10-fold serial dilutions of human genomic DNA.
(click image to enlarge)
Now real-time PCR methods can detect even smaller changes
and rare events with the new Promega GoTaq qPCR Master Mix.
The new GoTaq qPCR Master Mix frequently delivers earlier
cycle thresholds than SYBR® Green I. The advanced
functionality gives scientists both increased reliability
and reproducibility compared to the performance of existing
real-time reagent systems.
The GoTaq qPCR sensitivity comes from a novel fluorescent
dye that gives a brighter signal upon binding to
double-stranded DNA than SYBR Green I and is more resistant
to inhibitors. That means high performance even with low
expression targets.
Stability Facilitates Automation
GoTaq qPCR Master Mix offers enhanced stability at ambient
and elevated temperatures, making it ideal for automated
applications. After 24 hours at ambient temperature, GoTaq
qPCR Master Mix remains stable. Even after incubating GoTaq
at extreme temperatures (96° C) for 90 minutes, Cq values
remain unchanged. Other leading qPCR Master Mixes fail to
amplify after 30 minutes of exposure to elevated
temperatures.
Replaces SYBR
Set up for GoTaq is the same as SYBR Green l, utilizing the
same cycling conditions, and detection channel. GoTaq qPCR
Master Mix contains a low level of reference dye (CXR) that
can be detected using the same filters and setting as those
for ROX™. In addition, GoTaq qPCR Master Mix can be used
with any real-time instrument capable of detecting SYBR
Green I or FAM™ dye.
Through February 28, 2010 scientists are invited to take the
GoTaq qPCR Challenge. By submitting data comparing their
current real-time PCR reagents to GoTaq, scientist can
qualify for up 50% off for 1 year.
GoTaq is a registered trademark of Promega
Corporation. SYBR is a registered trademark of Molecular
Probes, Inc. FAM and ROX are trademarks of Applera
Corporation.
About Promega
Promega Corporation is a leader in
providing innovative solutions and technical support to the life
sciences industry. The company's 2,000 products enable
scientists worldwide to advance their knowledge in genomics,
proteomics, cellular analysis, molecular diagnostics and human
identification. Founded in 1978, the company is headquartered in
Madison, WI, USA with branches in 14 countries and over 50
global distributors. For more information about Promega, visit
www.promega.com
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