Press Releases
New Report Further Demonstrates Ultra-Glo™ Luciferase is Less Susceptible to Compound Interference
MADISON, WISCONSIN (March 17, 2009)
Research results just published in the Journal of
Medicinal Chemistry demonstrate the superior performance
of Promega Ultra-Glo™ Luciferase. The research, conducted by
the NIH Chemical Genomics Center (NCGC), describes Ultra-Glo
recombinant luciferase as approximately 90% less susceptible
to small molecule inhibition compared to another
commercially available luciferase. The NCGC is leading the
US government-sponsored efforts aimed at developing
biological probes and expanding the use of HTS assays in
academic settings.
The studies reveal why most screeners prefer Ultra-Glo as
the luciferase enzyme formulation in their screening and
profiling regimens. Ultra-Glo was derived by directed
evolution and is the key component in Kinase-Glo Assay and a
suite of other bioluminescent assays for high throughput
screening and profiling of small molecule compound
libraries.
At the 2007 SBS meeting in Montreal, Dr. Mohammed Kashem
from Boehringer Ingelheim Pharmaceuticals, first reported
similar findings when he compared Kinase-Glo and a second
commercially available ATP-detection luminescent reagent for
tolerance to interference by compounds in the BI screening
deck. When asked to comment on the NCGC findings, Dr. Kashem
replied, "The findings of Auld et al. are consistent with
ours (Kashem et al., SBS 2007 Poster #PST1C016) that
Kinase-Glo is much less prone to interference from library
compounds than PKLight reagent, making it a superior
ATP-detection reagent for identifying small-molecule
modulators of kinases by HTS."
About Promegaa
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providing innovative solutions and technical support to the life
sciences industry. The company's 2,000 products enable
scientists worldwide to advance their knowledge in genomics,
proteomics, cellular analysis, molecular diagnostics and human
identification. Founded in 1978, the company is headquartered in
Madison, WI, USA with branches in 14 countries and over 50
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