Figure #1. Schematic illustrating how the TNT T7 Insect
Cell Extract Protein Expression System functions.
Promega Corporation announces it has expanded its offerings
within its TNT® technology to include an insect expression
system and an improved system for expression in bacteria. The
recent additions give scientists the most comprehensive offering
of cell-free expression tools allowing the expression of proteins from
DNA in a single reaction.
The latest addition of an insect lysate system delivers unique
advantages, most importantly expression at a lower temperature
than other systems. Working in lower temperatures can enhance
protein folding and stability and is often the preferred method
for scientists performing mutagenesis studies.
The TNT system is a single step process that can be done in a
fraction of the time of the other multi-stepped offerings in the
market. The proprietary TNT technology from Promega utilizes DNA
as template for the coupled transcription/translation, and saves
researchers from purifying and quantifying mRNA for in vitro
expression, greatly streamlining the process. (Figure #1)
“Expanding applications have placed demands on performance for
protein expression in vitro in terms of yield and consistency,”
says Gary Kobs, product manager for Promega’s cell-free
expression systems. “Since its launch in 1992, the primary
application for TNT has evolved from confirming protein
expression to examining protein-protein interactions. As a tool
for protein-protein interaction, we know scientists not only
want greater yield, but also need to express proteins in a
variety of systems such as insect cell extract.”
Cell-free protein expression offers unique advantages when
compared to cell-based protein expression including time savings
and increased overall yields of functional, soluble, full–length
proteins. Typical reactions produce sufficient quantities of
protein that can be used for a variety of applications including
protein:protein and protein:nucleic acid interactions.
For more information regarding Promega’s cell-free protein
expression systems visit:
http://www.promega.com/applications/prtn_exp/
About Promega
Promega Corporation is a leader in providing innovative solutions and technical support to the life sciences industry. The company's 2,000 products enable scientists worldwide to advance their knowledge in genomics, proteomics, cellular analysis, molecular diagnostics and human identification. Founded in 1978, the company is headquartered in Madison, WI, USA with branches in 14 countries and over 50 global distributors. For more information about Promega, visit
www.promega.com.
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