The automated Differex™ method uses DNA IQ™ Resin to cap the sperm pellet after differential lysis and centrifugation. The sperm pellet is immobilized using paramagnetic particles. This allows the pellet to be washed via a robotic platform.
Processing time for 40 samples using traditional manual methods
typically requires a total of 3-5 days. By contrast, the
automated Differex method makes it possible to process up to 40
differential extraction samples in less than 5 hours, reducing
total processing time by over 70%. Once the differential
extraction has been completed, samples can move seamlessly
through extraction and purification using DNA IQ™ extraction
methods.
Currently the majority of backlogged samples are sexual assault
samples. They are particularly challenging to process as the
combined male and female DNA must be separated through
differential extraction prior to STR analysis and
interpretation. Until now, the commonly used methods have
required the use of harsh and dangerous chemicals coupled with a
time consuming manual regimen of washing, spinning and
incubation steps. The automated Differex method relies on
differential lysis of the epithelial fraction followed by sperm
pellet immobilization using paramagnetic particles. This allows
the pellet to be washed via a robotic platform.*
Differex methods are now available for three leading automation
platforms in forensics: the Beckman Coulter Biomek® 2000, Biomek
3000 and Tecan Freedom EVO® Platforms. System validation and
ongoing technical support are offered by the Promega team of
automation specialists. Laboratories using automated instrument
platforms in combination with Differex will be able to make
significant progress towards reducing their backlog of sexual
assault samples. Learn more about automated Differex at
www.promega.com/autodifferex/.
* Patent pending.About Promega
Promega Corporation is a leader in providing innovative solutions and technical support to the life sciences industry. The company's 2,000 products enable scientists worldwide to advance their knowledge in genomics, proteomics, cellular analysis, molecular diagnostics and human identification. Founded in 1978, the company is headquartered in Madison, WI, USA with branches in 14 countries and over 50 global distributors. For more information about Promega, visit
www.promega.com.
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