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Scientists can now save time and simplify the cloning process with the new Promega Thermosensitive Alkaline Phosphatase (TSAP). For dephosphorylation of DNA in cloning reaction, scientists get both speed and simiplicity because two unique features found in TSAP:
- TSAP is active in all restriction enzyme buffers tested. That eliminates cleanup and buffer swaps. Most importantly, it allows restriction and dephosphorylation in the same reaction at the same time.
- TSAP is irreversibly inactivated by heating at 74°C for 15 minutes. This
allows streamlining of the restriction enzyme digestion, desphosphorlyation and ligation procedure by eliminating the need of cleanup after alkaline phosphatase treatment.
As a quality product, TSAP is blue/white cloning-qualified. Scientists get a higher level of quality control for enzymes used in cloning applications. TSAP catalyzes the removal of 5' phosphate groups from DNA, thus preventing the recircularization and religation of linearized cloning vector DNA during ligation. It is effective on 3' and 5' overhangs and on blunt ends.
Promega catalog number M9910 contains 100 reactions of TSAP.
For more information or to request a sample visit:
www.promega.com/tsap/
Images of data available upon request.
About Promega
Promega Corporation is a leader in providing innovative solutions and technical support to the life sciences industry. The company's 1,450 products enable scientists worldwide to advance their knowledge in genomics, proteomics, cellular analysis, molecular diagnostics and human identification. Founded in 1978, the company is headquartered in Madison, WI, USA with branches in twelve countries and 54 global distributors. Annual sales exceed $190 million.
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