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Issue 100
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TITLE
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PDF (size)
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| Promega Notes - Tech Toons |
4.5Mb |
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Getting the Most from Your Transfections: Increasing Throughput and Sensitivity |
133kb |
|
Transfection-Quality Plasmid DNA in as Little as Ten Minutes
Using the PureYield™ Plasmid Miniprep System |
186kb |
| The S30 T7 High-Yield Protein Expression System |
115kb
|
| Cell-Free Protein Expression with the TNT® T7 Insect Cell Extract Protein Expression System |
115kb |
| Expression of Fusion Proteins: How to Get Started with the HaloTag® Technology |
104kb |
| Achieve the Protein Expression Level You Need with the Mammalian
HaloTag® 7 Flexi® Vectors |
143kb |
| Expanding Applications of Bioluminescence Technology: Predictive
Bioassays for TNFα Biologicals Potency and Dose-Standardization
Studies |
114kb |
| Luminogenic Enzyme Substrates: The Basis for a New Paradigm in
Assay Design |
108kb |
| Novel Biosensors to Monitor Cellular Events in Live Cells |
148kb |
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Issue 99
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TITLE
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PDF (size)
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|
Improve Protein Analysis with the New, Mass Spectrometry-Compatible ProteaseMax™ Surfactant |
393kb |
| Get the Convenience of Hot-Start PCR with the New GoTaq® Hot Start Polymerase |
298kb
|
| RNA Purification Kit Comparison: Yield, Quality and
Real-Time RT-PCR Performance |
330kb |
| MicroRNA Biosensors: Application for the psiCHECK™-2 Vector |
169kb |
| Use of the BacTiter-Glo™ Microbial Cell Viability Assay to Study Bacterial Attachment in Biofilm Formation |
186kb |
| NIH Chemical Genomics Center: Small-Molecule Screening for Investigating Fundamental Biological Questions |
73kb |
| Measuring Cell Health and Viability Sequentially by
Same-Well Multiplexing Using the GloMax®-Multi
Detection System |
430kb |
| Rat Hepatocyte Culture Physiology Shows Enhanced Cytochrome P450 Activity on a Synthetic Extracellular Matrix |
181kb |
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-top of page- |
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Issue 98
|
TITLE
|
PDF (size)
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|
GloMax®-Multi Detection System: Luminescence, Fluorescence and Absorbance in One Instrument |
1,027kb |
| [U-13C, 15N] Protein Labeling Using Escherichia coli Strain KRX |
449kb
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| Application of the BacTiter-Glo™ Assay for Rapid Enumeration and Screening of Antimicrobial Compounds for Mycobacterium avium Complex Bacteria |
109kb |
| Have You Seen the Maxwell® 16 Applications Database? |
198kb |
| Perform Automated Nucleic Acid Purification Using the SV Systems and the epMotion® 5075 VAC Workstation |
960kb |
| KRX Autoinduction Protocol: A Convenient Method for Protein Expression |
318kb |
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Issue 97
|
TITLE
|
PDF (size)
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|
Personal Automation™ for Increased Productivity |
89kb |
| New Maxwell® 16 Instrument Accessories Increase
the Versatility of Your Maxwell® 16 System |
529kb
|
| Maxwell® 16 Buffy Coat Genomic DNA
Purification Application |
234kb |
| Maxwell® 16 Tissue and Cell Total RNA LEV Systems |
292kb |
| The Versatility of the Maxwell® 16 System
for Genomic DNA Extraction |
182kb |
| ATP Measurement as a Means for Directly Estimating Active Biomass |
150kb |
| HaloCHIP™ System: Mapping Intracellular Protein:DNA Interactions using HaloTag® Technology |
212kb |
| ProTEV Protease for Fusion Protein Processing |
156kb |
| Monitor GPCR Modulation of Cellular cAMP with an HTS, Bioluminescence-Based Assay |
254kb |
| 15N Labeling of Proteins Overexpressed in the Escherichia coli Strain KRX |
147kb |
| ONE-Glo™ Luciferase Assay System: New Substrate, Better Reagent |
184kb |
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Issue 96
|
TITLE
|
PDF (size)
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|
The Bioluminescence Advantage |
235kb |
| Deciphering the pGL4 Vector Code |
123kb
|
| New High-Throughput Technology for Screening Transcriptional Regulatory Elements and Gene Networks |
258kb |
| Identify P-glycoprotein Substrates and Inhibitors with the Rapid, HTS Pgp-Glo™ Assay System |
255kb |
| P450-Glo™ CYP3A4 Biochemical and Cell-Based Assays |
217kb |
| Measure Biological and Cellular Activities with GloMax™ Luminometers and Bioluminescent Assays from Promega |
83kb |
| Protein Expression in Less Time: A Short Induction Protocol for KRX |
120kb |
| Compatibility of Single Step (KRX) Competent Cells with MagneGST™ Pull-Down System |
128kb |
| Selenomethionine Protein Labeling Using the
Escherichia coli Strain KRX |
191kb |
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Issue 95
|
TITLE
|
PDF (size)
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|
Comparison of the Flexi® Vector Systems to the Gateway® Cloning System |
|
|
TSAP: A New Thermosensitive Alkaline Phosphatase |
139kb |
| PIASy Regulation of STAT5 Activity:
Different Reporter Vectors, Different Responses |
142kb
|
| A Novel System for Regulated Protein Expression in Mammalian Cells |
222kb |
| Maxwell® 16 Polyhistidine Protein Purification Kit: Automated Protein Purification with Maximum Performance and Convenience |
257kb |
| Cell Surface HaloTag® Technology: Spatial Separation
and Bidirectional Trafficking of Proteins |
272kb |
| HaloTag® Protein: A Novel Reporter Protein for Human Neural Stem Cells |
226kb |
| A Polyclonal HaloTag® Antibody for Western Blotting and Immunocytochemistry |
169kb |
| Bioluminescence Imaging of Live Trout for Virus Detection using EnduRen™ Live Cell Substrate |
89kb |
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Issue 94
|
TITLE
|
PDF (size)
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| High-Performance RNA Isolation Using
the Maxwell™ 16 Total RNA Purification Kit |
163kb |
| Cleanup of TRIzol® Reagent-Purified Total RNA
Using the PureYield™ RNA Midiprep System |
143kb |
| The Next-Generation Assay for Mammalian Protein Interactions: The CheckMate™/Flexi® Vector Mammalian Two-Hybrid System |
150kb
|
| Optimal Generation of Short RNAs Using
the T7 RiboMAX™ Express RNAi System |
105kb |
| Measurement of Three Proteasome Proteolytic Activities Using Luminescent Assays |
128kb |
| Monitor the Ratio of Live and Dead Cells
Within a Population: MultiTox-Fluor Multiplex Cytotoxicity Assay |
179kb |
| The Single Step (KRX) Competent Cells: Efficient Cloning and High Protein Yields |
142kb |
| Functional Protein Production in the TNT® SP6 High-Yield Protein Expression System |
161kb |
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-top of page- |
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Issue 93
|
TITLE
|
PDF (size)
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| HisLink™ Spin Protein Purification System: Maximum Versatility in a Small Package |
167kb |
| Detecting DNA-Binding Protein Activity using a Membrane-Based Approach |
172kb |
| The Flexi® Vector Systems: The Easy Way to Clone |
260kb
|
| The MAO-Glo™ Assay: A Bioluminescent-Coupled Assay for Monoamine Oxidase Activity |
321kb |
| TNT® SP6 High-Yield Protein Expression System: More Protein from a Coupled Transcription/Translation System |
238kb |
| Optimizing Kinase Assays for Ultrahigh-Throughput Profiling Using the Kinase-Glo® Plus Assay |
231kb |
| A Complete Solution for Measuring Biological and Cellular Activities: GloMax™ Luminometers and Bioluminescent Assays |
173kb |
| In the Literature: Recent citations using Promega products |
121kb |
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-top of page- |
|
Issue 92
|
TITLE
|
PDF (size)
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| High-Throughput Biology: Combining Chemistry and Technology for Faster Results |
118kb |
| High-Throughput Compound Profiling Using Tecan Instrumentation |
92kb |
| Ultrahigh-Throughput Compound Profiling Using Next-Generation Dispensing Technology |
221kb
|
| The Plexor™ Systems Provide Accurate Quantitation in Multiplex qPCR and qRT-PCR |
285kb |
| PureYield™ RNA Midiprep System: Isolating Pure Total RNA without DNase |
287kb |
| Introducing the Maxwell™ 16 Instrument: A Simple, Robust and Flexible Tool for DNA Purification |
240kb |
| HaloLink™ Resin: High Capacity, Specificity and Scalable Througput for Protein Analysis |
319kb |
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Issue 91
|
TITLE
|
PDF (size)
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| Automated High-Throughput Protein Purification with MagneHis™
Particles |
109kb |
| A Bioluminescent Assay for Calpain Activity |
104kb |
| Synthesizing Fluorescent Target cDNA for Microarray-Based
Transcriptional Profiling: Considerations Regarding Primers and
Template RNA |
488kb
|
| GoTaq® Green Master Mix: From Amplification to Analysis |
114kb |
| Metal Affinity Tag for Protein Expression and Purification
using the Flexi® Vectors |
107kb |
| Expression of Soluble Native Human Proteins in Cell-Free
Extracts |
123kb |
|
-top of page- |
|
Issue 90
|
TITLE
|
PDF (size)
|
| Plexor™ Technology: A New Chemistry for Real-Time PCR |
132kb |
| Pronto!™
Plus Systems: New Integrated Reagent Systems for Optimal Microarray Performance |
319kb |
| Measuring Renilla Luciferase Luminescence in Living Cells |
168kb
|
| HisLink™ 96 Protein Purification System: Fast Purification of Polyhistidine-Tagged Proteins |
174kb |
| Wheat Germ Extract Plus For Higher Protein Yields |
121kb |
| MagneSil® Genomic, Large Volume System, for Large-Sample Genomic DNA Isolation |
149kb |
| Using Bioluminescent Reporter Genes to Optimize shRNA Target Sites for RNAi of the
bcr/abl Gene |
119kb |
| RNA Interference in
Drosophila S2 Cells: Effect of dsRNA Size, Concentration and Exposure Time |
112kb |
| Citations Isolating Bacterial DNA with the Wizard® Genomic DNA Purification Kit |
56kb |
|
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|
Issue 89
|
TITLE
|
PDF (size)
|
| HaloTag™ Interchangeable Labeling Technology for Cell
Imaging, Protein Capture and Immobilization |
344kb |
| pGL4 Vectors: A New Generation of
Luciferase Reporter Vectors |
265kb |
| A New System for Cloning and Expressing Protein-Coding
Regions |
348kb
|
| GoTaq® Flexi DNA Polymerase:
Robust Performance with Magnesium Optimization |
191kb |
| GeneClip™ U1 Hairpin Cloning Systems for Expression of
Short Hairpin RNAs in vivo |
248kb |
| BacTiter-Glo™ Assay for
Antimicrobial Drug Discovery and General Microbiology |
212kb |
|
-top of page- |
|
Issue 88
|
TITLE
|
PDF (size)
|
| Quantitate Microbial Cells Using a Rapid and Sensitive
ATP-Based Luminescent Assay |
198kb |
| Fast, Reliable, High-Quality Midiprep Plasmid Purification
Using the PureYield™ Plasmid Midiprep System |
245kb |
| The MagZ™ System: His-Tagged Protein Purification without
Hemoglobin Contamination |
200kb
|
| In Vitro His-Tag Pull-Down Assay Using MagZ™ Particles |
165kb |
| Suppression of Caspase-3 Expression Using the psiSTRIKE™ hMGFP
Vector |
252kb |
| Luciferase: From Bugs to Biotech and Beyond |
152kb |
| Technically Speaking: TNT® Rabbit Reticulocyte Lysate Systems–Easy Protein Expression |
107kb |
|
-top of page- |
|
Issue 87
|
TITLE
|
PDF (size)
|
| The Use of
Bioluminescent Reporter Genes for RNAi Optimization |
228kb |
| DNA-Directed
RNA Interference: Hairpin Cloning and Expression Made Easy |
214kb |
| Introducing
siLentGene™-2 U6 Hairpin Cloning Systems |
192kb
|
| Correlation
of Caspase Activity and Chemo-Response in Epithelial Ovarian Cancer
Cell Lines |
180kb |
| Introducing
the Rapid Response™ Reporter Vectors |
208kb |
| Detection
of Protein:Protein Interactions Using the MagneGST™ Pull-Down System |
209kb |
| Yeast
Plasmid Isolation Using the Wizard® Plus SV
Minipreps DNA Purification System |
111kb |
| Efficient
Purification of His-Tagged Proteins from Insect and Mammalian Cells |
215kb |
| Technically Speaking:
Cell-Based Caspase Assays: Analyzing the Data |
172kb |
|
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|
Issue 86
|
TITLE
|
PDF (size)
|
| The Pronto!™ Plus System: Increasing Microarray
Reproducibility, Reliability and Ease of Use |
297kb |
| Purification of GST-Fusion
Proteins by Magnetic Resin-Based MagneGST™ Particles |
296kb |
| Introducing the HisLink™ Protein Purification Resin |
219kb
|
| Purification of In Vitro
Transcribed RNA Using the SV Total RNA Isolation System |
164kb |
| MagneSil® Total RNA mini-Isolation System |
237kb |
| CodeBreaker™ siRNA Transfection
Reagent: Efficient Transfection of siRNA Duplexes into Mammalian Cells |
138kb |
| FastBreak™ Cell Lysis Reagent for Protein Purification |
177kb |
| RNasin® Ribonuclease
Inhibitors: Superior Performance for All of Your RNA Analysis Needs |
163kb |
| Technically Speaking: Choosing the Right Protein
Purification System |
108kb |
|
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|
Issue 85
|
TITLE
|
PDF (size)
|
| High-Throughput DNA Fragment Purification Using the MagneSil®
Automated 384-Well Clean-Up Systems |
403kb |
| Automated 96-Well Purification of
Genomic DNA from Whole Blood |
400kb |
| Introducing Chroma-Luc™ Technology |
429kb |
| Produce Functional siRNAs and
Hairpin siRNAs using the T7 RiboMAX™ Express RNAi System |
176kb |
| Detecting UV Irradiation-Induced Apoptosis with the
Caspase-Glo™ 3/7 Assay |
410kb |
| Automation of Genomic DNA
Purification Using the MagneSil® KF, Genomic System |
255kb |
| Automating Promega Cell-Based Assays in Multiwell
Formats |
384kb |
| Increased Renilla Luciferase Sensitivity in the
Dual-Luciferase® Reporter Assay System |
238kb |
| Technically Speaking: Automated
Solutions Technical Support |
161kb |
|
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|
Issue 84
|
TITLE
|
PDF (size)
|
| siLentGene™ U6 Cassette RNA Interference System: Rapid Screening of siRNA Targets |
181kb |
| The T7 RiboMAX™ Express RNAi System: Efficient Synthesis of dsRNA for RNA Interference |
146kb |
| Monster Green™ Protein: A Brighter, Longer-Expressing Green Fluorescent Protein |
208kb |
| Introducing the Beta-Glo™ Assay System |
135kb |
| Automated System for Isolation of Poly(A)+ mRNA |
195kb |
| Quantitative, Real-Time RT-PCR Expression Using the SV 96 Total RNA Isolation System |
155kb |
| Rapid Detection and Quantitation of His-Tagged Proteins Purified by MagneHis™ Ni-Particles |
138kb |
|
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|
Issue 83
|
TITLE
|
PDF (size)
|
| MagneHis™ Protein Purification System: Purification of His-Tagged Proteins in Multiple Formats |
166kb |
| Automated Polyhistidine-Tagged Protein Purification Using the MagneHis™ Protein Purification System |
172kb |
| Introducing the CellTiter-Blue™ Cell Viability Assay |
156kb |
| Introducing the Kinase-Glo™ Luminescent Kinase Assay |
145kb |
| Wizard MagneSil Tfx™ System for the Purification of Transfection-Grade DNA |
125kb |
| Introducing GoTaq™ DNA Polymerase: Improved Amplification With a Choice of Buffers |
145kb |
| Expanding the Capabilities of Plant Genomic DNA Purification |
232kb |
| Automating the Wizard® Magnetic 96 DNA Plant System on Thermo Labsystems' Kingfisher® |
126kb |
| Technically Speaking: RNAi: RNA Interference |
102kb |
|
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|
Issue 82
|
TITLE
|
PDF (size)
|
| Wizard®
SV Gel and PCR Clean-Up System |
221kb |
Introducing the Wizard®
SV 96 PCR Clean-Up System |
275kb |
| Promega's SV Membrane
Technology: The Evolution of an Indispensable Laboratory Tool |
273kb |
Introducing the
CytoTox-ONE™ Homogeneous Membrane Integrity Assay |
218kb |
| In Vitro Toxicology
and Cellular Fate Determination Using Promega Cell-Based Assays |
178kb |
| Reviewed: The
Challenge of Using SNPs in the Understanding and Treatment of Disease |
131kb |
| Technically
Speaking: T-Vector
Cloning |
148kb |
|
-top of page- |
|
Issue 81
|
TITLE
|
PDF (size)
|
| CellTiter-Glo™ Luminescent Cell Viability Assay:
Fast, Sensitive and Flexible |
190kb |
| Apo-ONE™ Homogeneous Caspase-3/7 Assay: Robust,
High-Throughput Apoptosis Detection |
137kb |
| Introducing the Wizard® SV and SV 96 Genomic DNA
Purification Systems |
239kb |
| Indirect Fluorescent Labeling of Microarray
Targets Using ImProm-II™ Reverse Transcriptase |
368kb |
| Express More Functional Protein: TNT® Quick
Coupled Transcription/Translation Systems |
102kb |
| Use of the READIT® SNP Genotyping System to
Screen Mutations Generated Using the GeneEditor™ in vitro Site-Directed
Mutagenesis System |
109kb |
| Dual-Glo™ Luciferase Assay System: Convenient
Dual Reporter Measurements in 96- and 384-Well Plates |
192kb |
| Work Smarter Using Isoschizomers and Neoschizomers |
94kb |
| Technically Speaking: Cell Viability
Assays |
78kb |
|
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|
Issue 80
|
TITLE
|
PDF (size)
|
| High-Quality
In Vitro Expression Cloning Using the Gold TNT® T7 Express 96
System |
164kb |
| Fast
& Efficient Production of RNA with the T7 RiboMAX™ Express System |
139kb |
| Optimized
Gene Expression with the T7 Sample System |
134kb |
| MagneSil™
Paramagnetic Particles: A High-Throughput PCR Purification System for
Microarrays |
165kb |
| Using
ImProm-II™ Reverse Transcriptase to Construct cDNA Libraries |
123kb |
| Demonstration
of Immunohistochemical Staining Using Promega's Anti-ACTIVE®
and Apoptosis Antibodies |
235kb |
| Technically
Speaking: Microarrays |
79kb |
|
-top of page- |
Issue 79
|
TITLE
|
PDF (size)
|
| READIT™ SNP Genotyping
System: Accurate, Flexible and Easy |
146kb |
| New
Synthetic Renilla Gene and Assay System Increase Expression, Reliability and
Sensitivity |
280kb |
| AccessQuick™ RT-PCR
System: Simple, Stable and Sensitive |
180kb |
| Using
ImProm-II™ Reverse Transcription System for Coupled RT-PCR |
154kb |
| Automated Purification of
PCR Products Using MagneSil™ Paramagnetic Particles |
233kb |
| Automated
Plasmid Purification Using MagneSil™ Paramagnetic Particles |
135kb |
| Automated Genomic DNA
Purification Using the Wizard® Magnetic 96 DNA Plant System |
277kb |
| High-Throughput
Purification Using the SV 96 Total RNA Isolation System |
240kb |
| Apo-ONE™ Homogeneous
Caspase-3/7 Assay: Rapid Apoptosis Detection in High-Throughput Applications |
139kb |
| CellTiter-Glo™
Assay: Flexible Luminescent Cell Viability Assay |
147kb |
| Technically Speaking:
Apoptosis Detection |
104kb |
|
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Issue 78
|
TITLE
|
PDF (size)
|
| Automated
Isolation of Genomic DNA Using Promega's DNA Binding Plates on the Beckman Biomek®
2000 |
690kb |
| Performance Advantages
Designed into Promega's PCR Master Mix |
1,307kb |
| Detection
of Enteric Pathogenic Viruses in Shellfish by RT-PCR |
498kb |
| ELISA In Situ: A Sensitive
Tool for Detecting Release of Endogenous BDNF |
199kb |
| Technically
Speaking: Promega RT-PCR Systems Explained |
138kb |
|
-top of page- |
Issue 77
|
TITLE
|
PDF (size)
|
| The Poly(A) mRNA Detection
System |
211kb |
| RNasin®
Ribonuclease Inhibitor Part I: Characterization of the Protein |
165kb |
| RNasin®
Ribonuclease Inhibitor Part II: A Tale of Two Proteins |
221kb |
| Characterization of TNT®
T7 Quick for PCR DNA |
189kb |
| FluoroTect™
GreenLys In Vitro Translation Labeling System |
246kb |
| Technically Speaking:
Subcloning Plasmid DNA Constructs |
37kb |
|
-top of page- |
Issue 76
|
TITLE
|
PDF (size)
|
| Overview: New Tools for
Cell Signaling & Neuroscience |
64kb |
| Immunostaining
with the New Anti-ACTIVE® p38 Antibody |
531kb |
| Tools to Study the
Activation of CaM KII in Neuronal Functions |
237kb |
| CaspACE™
FITC-VAD-FMK In Situ Marker as a Probe for Flow Cytometry Detection of Apoptotic Cells |
322kb |
| Wizard®
Magnetic DNA Purification System for Food: Part I. DNA Isolation and Analysis of GMO Foods
by PCR |
226kb |
| Wizard®
Magnetic DNA Purification System for Food: Part II. Semi-Automated DNA Isolation and
Analysis of GMO Foods |
174kb |
| Technically Speaking:
Anti-ACTIVE® Antibodies and MAPK Signaling Pathways |
57kb |
|
-top of page- |
Issue 75
|
TITLE
|
PDF (size)
|
| Overview: High-Throughput Solutions |
64kb |
| Bright-Glo™
and Steady-Glo® Luciferase Assay Systems: Reagents for Academic and Industrial
Applications |
261kb |
| High-Throughput Solutions
for DNA Purification Using MagneSil™ Paramagnetic Particles |
473kb |
| A High-Throughput System
for Screening Potential Antiviral Compounds Using the CellTiter® AQueous
One Solution Assay |
285kb |
| Anti-ACTIVE®
Caspase-3 pAb for the Detection of Apoptosis |
365kb |
| CaspACE™ FITC-VAD-FMK
In Situ Marker for Apoptosis: Applications for Flow Cytometry |
536kb |
| Protein
Kinases as Drug Targets in High-Throughput Systems |
195kb |
| Using InCELLect™
Cell-Permeable, Stearated Peptides to Probe Cellular Signaling Reactions In Vivo |
131kb |
| A
Comparison of pCI-neo Vector and pcDNA4/HisMax Vector |
119kb |
| Technically Speaking: M-MLV
Reverse Transcriptase, RNase H Minus |
48kb |
|
-top of page- |
Issue 74
|
TITLE
|
PDF (size)
|
| Overview: Luciferase Lights
the Way |
77kb |
| Design
of Homogeneous Luciferase Assays |
149kb |
| The AttoPhos®
System for Fluorescent Detection of Alkaline Phosphatase in an Enzyme-Linked Assay |
121kb |
| A
New TNT® System for Enhanced Expression of PCR DNA |
166kb |
| The Y Chromosome Deletion
Detection System, Version 1.1 |
104kb |
| Ribo
m7G Cap Analog: A Reagent for Preparing In Vitro Capped Transcripts |
76kb |
| A Strategy for Survival:
The Akt Signaling Cascade |
77kb |
| Technically
Speaking: Ribo m7G Cap Analog |
52kb |
| The Wisconsin Youth
Apprenticeship Program in Biotechnology |
136kb |
| Spotlight:
Promega's Global Technical Services |
28kb |
|
-top of page- |
Issue 73
|
TITLE
|
PDF (size)
|
| Overview: The Biotechnology
Revolution |
477kb |
| The
DNAQuant™ DNA Quantitation System for Sensitive Detection of dsDNA |
121kb |
| Wizard® SV 96
Plasmid DNA Purification System: High Quality Plasmid DNA for Use in Fluorescent
Sequencing Methods |
131kb |
| Rapid
Partial Digestion of DNA Fragments by PCR with 5-methyl-dCTP |
400kb |
| Using the Access RT-PCR
System: Reaction Parameters That Affect Efficient Amplification |
121kb |
| Increasing
Yield with the Wizard® PureFection Plasmid DNA Purification System |
113kb |
| Using the Wizard®
Genomic DNA Purification Kit with 96 Well Plates |
151kb |
| Screening
for Genetically Modified Organisms in Food Using Promega's Wizard® Resin |
191kb |
| Constructing Genomic
Libraries Using the pGEM®-T Vector |
298kb |
| Technically
Speaking: Quantitating DNA |
61kb |
|
-top of page- |
Issue 72
|
TITLE
|
PDF (size)
|
| Overview: Signaling
"Circuitry" |
301kb |
| Death
Check™ Assay Systems for Monitoring Apoptosis |
862kb |
| Cleaved PARP as a Marker
for Apoptosis in Tissue Sections |
731kb |
| PC12
Cell Extracts for Use with the New Anti-pT183 MAPK and Anti-ACTIVE®
Antibodies |
990kb |
| Isolation and Analysis of
Nucleic Acids from Human Blood: A Molecular Diagnostics Application |
460kb |
| Technically
Speaking: Cell Proliferation and Apoptosis Detection |
52kb |
| FAQspeak: Frequently Asked
Questions of Promega's Technical Services Department |
270kb |
| Product
InCite: Articles Citing Promega Products in Novel and Interesting Applications |
369kb |
|
-top of page- |
Issue 71
|
TITLE
|
PDF (size)
|
| Isolation of RNA from
Pacific Oyster Larvae Using the SV Total RNA Isolation System |
221kb |
| U0126:
An Inhibitor of MKK/ERK Signal Transduction in Mammalian Cells |
241kb |
| Rapid Ligation for the pGEM®-T
and pGEM®-T Easy Vector Systems |
171kb |
| Cloning
Blunt-End Pfu DNA Polymerase-Generated PCR Fragments into pGEM®-T
Vector Systems |
208kb |
| Rapid Isolation of Genomic
DNA from Human Samples: Forensics and Molecular Diagnostics Applications |
225kb |
| Postmortem
Isolation and Analysis of Human Genomic DNA from HIV-Infected Tissues: A
Molecular Diagnostics Application |
180kb |
| TransFast™
Transfection Reagent Update |
305kb |
| FAQspeak: Frequently Asked
Questions of Promega's Technical Services Department |
190kb |
| Product
InCite: Articles Citing Promega Products in Novel and Interesting Applications |
197kb |
|
-top of page- |
Issue 70
|
TITLE
|
PDF (size)
|
| Applications of Promega's
In Vitro Expression Systems |
236kb |
| Steady-Glo®
Luciferase Assay System for High-Throughput Screening Applications |
244kb |
| Recombinant Human Mast Cell
Tryptase |
264kb |
| Development
of a Rapid Capture ELISA Using PCR Products and the PinPoint™ System |
274kb |
| Options for the Protein
Truncation Test Protocol: A Molecular Diagnostics Application |
264kb |
| Wizard®
Plus Minipreps for the Isolation of Binary Plasmids from Agrobacterium
tumefaciens |
197kb |
| Technically Speaking:
Eukaryotic Expression: Transfection and Reporter Vectors |
175kb |
| FAQspeak:
Frequently Asked Questions of Promega's Technical Services Department |
197kb |
| Product InCite: Articles
Citing Promega Products in Novel and Interesting Applications |
217kb |
|
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Issue 69
|
TITLE
|
PDF (size)
|
| DeadEnd™ Colorimetric
Apoptosis Detection System: Applications in Pathology |
690kb |
| U0126:
A Novel, Selective and Potent Inhibitor of MAP Kinase Kinase (MEK) |
203kb |
| New Anti-ACTIVE® MAPK
& 'pan ERK 1/2' Antibodies for Western Analysis |
241kb |
| MagneSil™
Paramagnetic Particles: Novel Magnetics for DNA Purification |
286kb |
| High Throughput Screening
for Cytotoxicity Using the CellTiter 96® AQueous One Solution Assay |
193kb |
| Isolating
Nucleic Acids from Small Amounts of Brain Lysates Using the SV Total RNA Isolation System |
198kb |
| Separate Isolation of
Genomic DNA and Total RNA from Single Samples Using the SV Total RNA Isolation System |
284kb |
| Technically
Speaking: Detecting Apoptosis |
167kb |
| FAQspeak: Frequently Asked
Questions of Promega's Technical Services Department |
197kb |
| Product
InCite: Articles Citing Promega Products in Novel and Interesting Applications |
186kb |
|
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Issue 68
|
TITLE
|
PDF (size)
|
| Wizard®
PureFection Plasmid DNA Purification System: The New Standard in Isolating Transfection
Grade Plasmid DNA |
310kb |
| Pfu
DNA Polymerase: A High Fidelity Enzyme for Nucleic Acid Amplification |
256kb |
| Primer Pairs for Mediator
and Receptor mRNA Analysis |
179kb |
| Assay
Systems for Detecting Apoptosis and Cell Death |
297kb |
| Quantitative RT-PCR: Rapid
Construction of Templates for Competitive Amplification |
286kb |
| Molecular
Diagnostics: Isolation and Analysis of Human Genomic DNA |
269kb |
| Molecular Diagnostics:
Isolation and Analysis of RNA from Human Blood |
377kb |
| Isolation
of RNA from Plant, Yeast and Bacteria |
179kb |
| Isolation of Spirochete DNA
Using the Wizard® Genomic DNA Purification Kit |
178kb |
| Optimized
Cloning with T Vectors |
170kb |
| Frequently Asked Questions
of Promega's Technical Services Department |
201kb |
| Articles
Citing Promega Products in Novel and Interesting Applications |
188kb |
| Promega's GenePrint®
PowerPlex® System Receives "Top 100 Innovative Products" Award from R&D
Magazine |
286kb |
|
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Issue 67
|
TITLE
|
PDF (size)
|
| In Vitro
Expression Cloning Using the TNT® Coupled Reticulocyte Lysate
System |
199kb |
| A
General Method for Isolating Targets of RNA and DNA Binding Proteins |
264kb |
| Anti-ACTIVE® Antibody
for Specific Detection of Phosphorylated CaM KII Protein Kinase |
288kb |
| Inhibition
of EGF-Induced ERK1 and ERK2 Enzyme Activation in NIH3T3 Cells by In Situ
Electroporation of a Nonpermeant Inhibitor |
451kb |
| Technically Speaking: SV
Total RNA Isolation System |
217kb |
| Articles Citing Promega
Products in Novel and Interesting Applications |
200kb |
|
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Issue 66
|
TITLE
|
PDF (size)
|
| The CheckMate™
Mammalian Two-Hybrid System |
243kb |
| CaspACE™
Assay System, Fluorometric |
199kb |
| Application
of the TNT® T7 Quick System to Selection and Evolution of
Antibody Combining Sites |
223kb |
| Streptavidin MagneSphere®
Paramagnetic Particles |
158kb |
| Frequently
Asked Questions of Promega's Technical Services Department |
322kb |
| Articles Citing Promega
Products in Novel and Interesting Applications |
268kb |
|
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Issue 65
|
TITLE
|
PDF (size)
|
| An Efficient New
Transfection Reagent for Eukaryotic Cells: TransFast™ Transfection Reagent |
295kb |
| T4
RNA Ligase: A Molecular Tool for RNA and DNA Manipulations |
176kb |
| Rapid Luciferase Reporter
Assay Systems for High Throughput Studies |
257kb |
| The
Chemistry of Bioluminescent Reporter Assays |
328kb |
| Tfx™-50 Reagent and
High Efficiency Transfection of Vascular Tissue In Vitro and In Vivo |
476kb |
| Stability
of pGEM®-T Vectors |
171kb |
| Betaine and DMSO: Enhancing
Agents for PCR |
197kb |
| GeneEditor™
In Vitro Site-Directed Mutagenesis System |
191kb |
|
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Issue 64
|
TITLE
|
PDF (size)
|
| Advances in the SAM2®
Membrane Technology: High Throughput Biotin Capture Systems for Use in Rapid Screening |
221kb |
| RNA
Purification: A Rapid and Versatile Protocol for the Isolation of Total RNA |
246kb |
| Measuring Apoptosis in
Individual Cells with the Comet Assay |
252kb |
| Signal
Integration and Activation of MAPK, JNK and p38 Kinase Pathways in 293 Cells |
212kb |
| In Vitro Transcription
on DNA Templates Immobilized to Streptavidin MagneSphere® Paramagnetic
Particles |
277kb |
| A
Comparison of Western Blotting Techniques Using Phospho-Specific Antibody Detection of
Protein Kinase Activation |
231kb |
| Technically Speaking:
Anti-ACTIVE® Antibodies to MAPK, JNK and p38 |
158kb |
| Promega's
Dual-Luciferase® Reporter Assay System Receives "Top 100 Innovative
Products" Award from R&D Magazine |
210kb |
|
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Issue 63
|
TITLE
|
PDF (size)
|
| Detection of JNK, p38 and
MAPK Enzyme Activation Using Anti-Dual-Phosphopeptide Antibodies: Coordinated Signaling of
the Extracellular Signal-Regulated Protein Kinase (ERK) Superfamily |
256kb |
| SignaTECT®
DNA-Dependent Protein Kinase Assay System |
175kb |
| Primer Pairs for
Neurotrophic Factor mRNA Analysis |
195kb |
| Transfection
of Insect Cells with Tfx™-20 Reagent |
220kb |
| Technically Speaking: Tips
for Working with RNA & Troubleshooting Downstream Applications |
183kb |
|
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Issue 62
|
TITLE
|
PDF (size)
|
| The GeneEditor™
In Vitro Mutagenesis System: Site-Directed Mutagenesis Using Altered β-Lactamase
Specificity |
222kb |
| PCR
Core Systems: Complete Reagent Systems for DNA Amplification |
180kb |
| The Protein Truncation Test
(PTT) |
183kb |
| Rapid
Isolation of RNA from Small Quantities of Human Whole Blood for use in RT-PCR Analysis |
224kb |
| Literature Reviews |
144kb |
| Cloning
Blunt-End DNA Fragments Into the pGEM®-T Vector Systems |
229kb |
| Technically Speaking: DNA
Amplification: Preparation of Template and Recovery of Product |
163kb |
|
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Issue 61
|
TITLE
|
PDF (size)
|
| A Comparative Study of T7
RNA Polymerase Quality |
220kb |
| Vendor
Comparison: In Vitro Transcription Systems |
258kb |
| Site-Directed Mutagenesis |
298kb |
| Altered
Sites® II In Vitro Mutagenesis Systems |
182kb |
| AgarACE®
Agarose-Digesting Enzyme Part Three: DNA/RNA Recovery and Applications Testing for Use in
Translation, Transcription/Translation, Prime-a-Gene® Labeling and Fluorescent
DNA Sequencing |
311kb |
| Amplification
of Flanking Regions: New Applications and Performance Optimization of Single Specific
Primer PCR and T-Vector Cloning |
207kb |
| A Comparison of
Ribonuclease Inhibitors |
202kb |
| Technically
Speaking: RNAgents® Total RNA Isolation System |
177kb |
|
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Issue 60
|
TITLE
|
PDF (size)
|
| Improved PCR Amplification
Using TaqBead™ Hot Start Polymerase |
262kb |
| Applications
of the TNT® T7 Quick Coupled Transcription/Translation System |
287kb |
| PolyATtract®
Systems for mRNA Purification |
238kb |
| In
Vitro Transcription from Biotinylated DNA Immobilized on Streptavidin MagneSphere®
Paramagnetic Particles |
184kb |
| Digestion of PCR and RT-PCR
Products With Restriction Endonucleases Without Prior Purification or Precipitation |
212kb |
| BSA
and Restriction Enzyme Digestions |
144kb |
|
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|
Issue 59
|
TITLE
|
PDF (size)
|
| Detection
and Quantitation of Protein Tyrosine Kinases |
310kb |
| Wizard®
Plus SV Minipreps DNA Purification System: The Next Generation in
Miniprep Purification |
376kb |
| Improved
Non-Radioactive Assay to Measure Cellular Proliferation or Toxicity: The
CellTiter 96® AQueous One Solution Cell
Proliferation Assay |
200kb |
| A
Trio of Tfx™ Transfection Reagents for Eukaryotic Cells |
303kb |
| Analysis
of DNA Fragmentation in Epidermal Keratinocytes Using the Apoptosis
Detection System, Fluorescein |
455kb |
| MAP
Kinase Signaling Pathways |
230kb |
| Detection
of MAP Kinase Activation Using Anti-ACTIVE® MAPK pAb |
257kb |
| BSA
and Restriction Enzyme Digestions |
317kb |
| Technically
Speaking: CellTiter 96® Non-Radioactive Cell Proliferation
Assays and CytoTox 96® Non-Radioactive Cytotoxicity Assay |
158kb |
|
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|
Issue 58
|
TITLE
|
PDF (size)
|
| pTARGET™
Vector: A New Mammalian Expression T-Vector |
246kb |
| The
TNT® T7 Quick Coupled Transcription/Translation
System |
209kb |
| New
Approaches to DNA Fingerprint Analysis |
595kb |
| SAM2®
Biotin Capture Membrane and SignaTECT® Protein Kinase Assay
Systems |
246kb |
| Mutagenesis
and Mammalian Expression Using the Altered Sites® II Mammalian
Mutagenesis System |
204kb |
| Specific
Detection of Biologically Active MAP Kinase Using Anti-ACTIVE®
MAPK pAb |
190kb |
| The pGEM®-T
and pGEM®-T Easy Vector Systems |
192kb |
| Purification
of Fragmented Apoptotic DNA Using the Wizard® Plus
Series 9600™ DNA Purification System |
209kb |
| Technically
Speaking: Access RT-PCR System |
174kb |
|
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|
Issue 57
|
TITLE
|
PDF (size)
|
| Dual-Luciferase®
Reporter Assay: An Advanced Co-Reporter Technology Integrating Firefly and
Renilla Luciferase Assays |
252kb |
| Detection
of Apoptotic Cells Using the Apoptosis Detection System, Fluorescein |
377kb |
| A
Convenient Non-Radioactive Assay for the CAT Reporter Gene Using the CAT
ELISA System |
217kb |
| Detection
of the Bcr-Abl Gene in CML/ALL Patients |
177kb |
| cDNA
Synthesis Using the Universal RiboClone® System |
165kb |
| Application
of Promega's TNT® T7 Coupled Reticulocyte
Lysate System in the Study of Fas-Associated Apoptosis |
186kb |
| Technically
Speaking: Wizard® Plus DNA Purification Systems |
153kb |
|
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|
Issue 56
|
TITLE
|
PDF (size)
|
| Rapid
Isolation of High Quality Genomic DNA from Various Sources Using the
Wizard® Genomic DNA Purification Kit |
189kb |
| Tfx™-50
Reagent Increases the Uptake of Oligonucleotides by Leukemic Cells |
241kb |
| Use
of Promega's CytoTox 96® Non-Radioactive Cytotoxicity Assay
for the Study of MHC Class I Presentation of an Exogenous Antigen |
171kb |
| Technically
Speaking: PolyATtract® mRNA Isolation Systems |
157kb |
| RNasin®
Ribonuclease Inhibitor: Celebrating 15 Years of RNase Inhibition |
228kb |
|
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|
Issue 55
|
TITLE
|
PDF (size)
|
| CAT
Reporter Systems: New pCAT®3 Reporter Vectors and Antibodies
Provide Increased Expression and Detection Capabilities |
345kb |
| A
Rapid Protocol for the Isolation of Total RNA Suitable for RT-PCR |
274kb |
| Wizard®
Series 9600™ DNA Purification System Enhancements |
423kb |
| DNA
Excision Repair Catalyzed In Vitro by the HeLaScribe® Nuclear
Extract |
253kb |
| pGEM®-T
Vector: Cloning of Modified Blunt-Ended DNA Fragments |
218kb |
| Technically
Speaking: TGFß1 ELISA System |
219kb |
|
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|
Issue 54
|
TITLE
|
PDF (size)
|
| Isolation
of Total RNA and mRNA from Plant Tissues |
255kb |
| Simple
Affinity Purification Using a T-Vector for In Vivo Biotinylation:
Applications for Antibody Purification and Protein-Protein Interaction
Studies |
253kb |
| AgarACE®
Agarose-Digesting Enzyme Part Two: Applications Testing for Cloning,
Sequencing and PCR |
317kb |
| Luciferase
Assay System Vendor Comparison |
246kb |
| T4 DNA
Polymerase |
270kb |
| EGGstract®
IgY Purification System |
148kb |
|
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|
Issue 53
|
TITLE
|
PDF (size)
|
| A
Sensitive Single-Tube, Two-Enzyme System for RT-PCR |
260kb |
| AgarACE®
Agarose-Digesting Enzyme Part One: Description of the Enzyme and its
Performance in Agarose Hydrolysis |
275kb |
| Effects
of Bacterial Strains Carrying the endA1 Genotype on DNA Quality
Isolated with Wizard® Plasmid Purification Systems |
398kb |
| The
ISOQUANT® Protein Deamidation Detection Kit |
268kb |
| Applications
of the TNT® Coupled Transcription/Translation
Systems |
261kb |
| Generation
of Nested Deletions Using the Erase-A-Base® System |
211kb |
| Detection
of RNA Polymerase II in Mammalian Species by Western Blot Analysis |
206kb |
|
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|
Issue 52
|
TITLE
|
PDF (size)
|
| Tfx™-50
Reagent: A New Transfection Reagent for Eukaryotic Cells |
323kb |
| Use
of a 96 Well Format for Small Scale mRNA Isolation and cDNA Synthesis |
462kb |
| Survey of
Restriction Enzyme Quality |
243kb |
| Protein
Quantitation Using the Quantify™ Protein Assay System |
262kb |
| Immunohistochemistry
Using Anti-TGFß1 Polyclonal Antibody |
266kb |
| Deamidation
and Isoaspartate Formation in Proteins |
265kb |
| Fluorescent
DNA Sequencing Using Templates Purified with the Wizard® 373
DNA Purification System |
563kb |
|
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|
Issue 51
|
TITLE
|
PDF (size)
|
| The pCI-neo
Mammalian Expression Vector |
280kb |
| Neurotrophic
Factors Part Two: Their Role in Development, Trauma and Disease |
225kb |
| Using
Promega's CytoTox 96® Non-Radioactive Cytotoxicity Assay to
Measure Cell Death Mediated by NMDA Receptor Subunits |
225kb |
| pGEM®
T-Vector System |
152kb |
|
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|
Issue 50
|
TITLE
|
PDF (size)
|
| Increased
Expression & Convenience with the New pGL3 Luciferase Reporter Vectors |
401kb |
| RecA
Cleavage and Protection for Genomic Mapping and Subcloning |
296kb |
| Simplified
Protocols for Using RecA Cleavage and Protection to Target Restriction
Enzyme Action |
241kb |
| Neurotrophic
Factors Part One: An Overview |
243kb |
| Signal
Transduction of Neurotrophin Receptors: Basic Concepts and Available
Pharmacological Tools |
211kb |
| Efficient
Purification of Multiple PCR Reactions or DNA Fragments from Agarose |
316kb |
| FluoroAmp™
Oligonulcleotide Non-Isotopic Labeling Systems |
243kb |
| Riboprobe®
and RiboMAX™ In Vitro Transcription Systems |
153kb |
|
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