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Rat Hepatocyte Culture Physiology Shows Enhanced Cytochrome P450 Activity on a Synthetic Extracellular Matrix
Mark E. Rothenberg1, James J. Cali2,
Mary Sobol2, Michael W. Briggs1 and Todd Upton1
1Technical Applications, Corning Life Sciences, 2Promega
Corporation
We examined the impact of a new three-dimensional synthetic matrix on
hepatocyte physiology. Hepatocytes were plated on Corning labware with
Ultra-Web™ and Ultra-Web™ polyamine surfaces and on Becton Dickinson
(BD) BioCoat™ collagen I microplates. The samples then were challenged
for 48 hours with two known modulators of cytochrome P450, and P450
activity was measured using the P450-Glo™ Assay. The data show enhanced
physiologic function of primary rat hepatocytes on microplates coated
with the Ultra-Web™ synthetic matrix.
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