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RNA Purification Kit Comparison: Yield, Quality and
Real-Time RT-PCR Performance
Trista Schagat1, Lisa Kiak2 and Michelle Mandrekar1
1Promega Corporation, 2Wyeth Pharmaceuticals
Promega offers total RNA isolation kits in a range of formats designed to meet different sample size and throughput needs. We compared the yield and quality of RNA from these kits to RNA isolated using Qiagen and Invitrogen kits. We also compared RNA performance in real-time RT-PCR. These data highlight the quality of Promega kits and utility in gene expression profiling. By comparing a breadth of kits, we found there can be kit-dependent differences in gene expression levels detected by real-time RT-PCR, and this should be considered when making quantitative RT-PCR comparisons.
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