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KRX Autoinduction Protocol: A Convenient Method for Protein
Expression
Trista Schagat, Rachel Ohana, Paul Otto, Jim Hartnett and Michael Slater
Promega Corporation
Recombinant protein expression in bacteria is a simple, yet time-consuming
process. Typically cells are grown overnight to stationary phase,
diluted and grown to an optimal density, then induced. To simplify this
process, we took advantage of the tight regulation of protein expression
by glucose and rhamnose in the KRX strain of E. coli. We
developed an autoinduction protocol that eliminates the need for culture
density monitoring and a separate induction step. We describe early and
late autoinduction protocols in LB medium and demonstrate autoinduced
expression of three different proteins.
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