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Perform Automated Nucleic Acid Purification Using the
SV Systems and the epMotion® 5075 VAC Workstation
Sarah Shultz and Cristopher Cowan
Promega Corporation
Automating nucleic acid purification from a variety of sample types is
helpful for many high-throughput applications. Automated sample
processing must be timely, precise and consistent, producing product
that is free from cross-contamination, pure from other contaminants and
of an expected yield for direct use in downstream applications. Here we
present fast and easy-to-use nucleic acid isolation methods on the
Eppendorf epMotion® 5075 VAC liquid handling
workstation. The Wizard® SV 96 Genomic DNA Purification
System and the SV 96 Total RNA Isolation System are used on the epMotion®
5075 VAC workstation to successfully isolate genomic DNA and total RNA
from mouse tails and tissue culture cells with results comparable to
manual and other automated isolation techniques.
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