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Application of the BacTiter-Glo™ Assay for Rapid Enumeration
and Screening of Antimicrobial Compounds for Mycobacterium avium
Complex Bacteria
Alice Yuroff1, Frank Fan2, Braeden Butler2 and
Michael Collins1
1Department of Pathobiological Sciences,
University of Wisconsin-Madison, 2Promega Corporation
Experiments were performed to determine if the BacTiter-Glo™ Microbial
Cell Viability Assay could be used as a rapid method to count
Mycobacterium avium complex (MAC) organisms. Using the BacTiter-Glo™
Assay, the amount of ATP measured was linear (r2 < 0.99) with
respect to MAC cell number between 107 and 103
cells; the limit of detection was 104–103 cells
per 100 µl. Freezing cell suspensions at –80 °C prior to the assay
significantly increased the luminescent signal in the assay. Exposure to
antimicrobial compounds azithromycin and chlorine resulted in a
significant decrease in both viable cell numbers and ATP levels.
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