|
Download
article
449kb
pdf? |
|
[U-13C, 15N] Protein Labeling
Using Escherichia coli Strain KRX
Kate Qin Zhao1, Marco Tonelli2,
Jim Hartnett1 and Michael R. Slater1
1Promega Corporation, 2NMRFAM, University of
Wisconsin-Madison
We have developed a protocol for efficient [U-13C,
15N]-labeled protein production in the highly competent E. coli
strain, KRX, using Silantes CN OD2 medium. This procedure can facilitate
protein analysis by NMR spectroscopy and other methodologies that
require 13C and 15N protein labeling.
|
