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15N Labeling of Proteins Overexpressed
in the Escherichia coli Strain KRX
Kate Qin Zhao, Jim Hartnett, and Michael R. Slater
Promega Corporation
We describe a protocol for efficient 15N-labeled protein production in the E. coli strain, KRX. This procedure can help streamline
the process of target cloning, protein expression, screening and preliminary protein folding/aggregation assessment by NMR spectroscopy
and other methods that require 15N protein labeling.
Correction: Figure 2, Panel B, is incorrectly labeled on the X axis. The correct X axis label is
1H (ppm). An Erratum from Promega Notes 98 showing the
corrected figure is attached as the last page of the article pdf.
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