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Monitor GPCR Modulation of Cellular cAMP with an HTS,
Bioluminescence-Based Assay
Said A. Goueli, Kevin Hsaio, and Jolanta Vidugiriene
Promega Corporation
The cAMP-Glo™ Assay is a bioluminescence assay for monitoring changes in intracellular cAMP concentrations with high
sensitivity (30 fmol ± 5 SEM cAMP/well) and reproducibility (Z´ >0.8). This assay can be used to measure cAMP concentration in a singletube
or high-throughput-screening (HTS) format and is less prone to compound interference than fluorescence-based assays.We have used
the cAMP-Glo™ Assay to generate EC50 values for agonists and IC50 values for antagonists that modulate GPCRs that alter adenylate cyclase
activity (Gαs, and Gαi).The cAMP-Glo™ Assay is compatible with adherent, suspension, and frozen cells, offering flexibility and convenience
to the researcher, and the cAMP-Glo™ Assay can be used to determine cAMP concentration in tissue extracts.
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