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Selenomethionine Protein Labeling Using the Escherichia coli Strain KRX
Kate Qin Zhao, Ph.D., Jim Hartnett, M.S., and Michael R. Slater, Ph.D.
Promega Corporation
With Monster Green® fluorescent protein as a model, we can achieve an average of 90% or greater selenomethionine
(Se-Met) incorporation when the GFP is expressed in the E. coli strain KRX. Therefore, it is feasible to produce Se-Met-labeled protein samples for structural biology applications, such as X-ray crystallography, using the Single Step (KRX) Competent Cells.
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