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Compatibility of Single Step (KRX) Competent Cells with the MagneGST™ Pull-Down System
Trista Schagat, Ph.D.
Promega Corporation
This study demonstrates the use of Single Step (KRX) Competent Cells for studying protein:protein interactions using the MagneGST™ Pull-Down System. GST-fusion
bait protein expressed in KRX was captured on MagneGST™ glutathione-linked magnetic particles and incubated with prey protein expressed in vitro in the TNT®
T7 Quick Coupled Transcription/Translation System. Captured prey was detected using HaloTag® Interchangeable Labeling Technology.
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