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Protein Expression in Less Time: A Short Induction Protocol for KRX
Lynn Litterer, Ph.D., and Trista Schagat, Ph.D.
Promega Corporation
Single Step (KRX) Competent Cells are ideal for rapidly generating new clones for recombinant protein expression.
We examined the effect of induction time and media on recombinant protein yield and activity in KRX. Firefly and Renilla luciferases
were used as models. Induction times as brief as 4 hours were sufficient to produce significant quantities of active protein. Using Single Step (KRX) Competent Cells
with this short induction protocol, protein can be expressed and analyzed in less time, without the need for overnight induction.
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