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Identify P-glycoprotein Substrates and Inhibitors with the Rapid, HTS Pgp-Glo™ Assay System
Dongping Ma, M.S., and James J. Cali, Ph.D.
Promega Corporation
The Pgp-Glo™ Assay Systems are luminescent P-glycoprotein (Pgp) ATPase
assays. The Pgp-Glo™ Assay detects the effects of compounds on
recombinant human Pgp in a cell membrane fraction. The assay couples the
ATPase activity of Pgp to the ATP dependence of the light-generating
reaction of firefly luciferase. The rate of ATP consumption by Pgp is
inversely correlated with the light output of the luciferase reaction.
The Pgp-Glo™ Assay is easily amenable to high-throughput screening and
can complement cell-based Pgp studies.
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