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Deciphering the pGL4 Vector Code

The pGL4 Vectors are the most advanced luciferase reporter gene vectors that maximize expression, minimize off-target responses such as anomalous expression, and respond rapidly to transcriptional dynamics. pGL4 Vectors that express firefly luciferase or Renilla luciferase are available. The pGL4 Vectors offer a variety of choices for three key components: The luciferase reporter gene, the upstream elements, including any promoters and response elements, and the selectable marker. The vector nomenclature for the pGL4 Vectors logically reflects these features. As an example, we will decipher the name of the pGL4.31[luc2P/CRE/Hygro] Vector.

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Promega Notes 96 (2007) 6–7: Request this issue.
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