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HaloTag® Protein: A Novel Reporter Protein for Human Neural Stem Cells
Soshana Svendsen, Ph.D.1, Erin
McMillan, B.S.2, Chad Zimprich, B.S.1, Clive
N. Svendsen, Ph.D.2, Georgyi V. Los, Ph.D.1
1Promega Corporation, 2University of
Wisconsin-Madison
Stem cells and related proteins are the focus of many scientific studies.
The ability to label specific proteins within stem cells may provide
important information about protein dynamics and function. Here we use a
technology to covalently tether different wavelength fluorophores to a
specially designed reporter, the HaloTag® protein. The HaloTag® protein,
either alone or fused to a protein of interest, was expressed in human
neural stem cells (hNSC) and successfully labeled with several HaloTag® Ligands. The different colored cell-impermeant and cell-permeant
fluorophores showed spatial separation and real-time translocation of
membrane and internal protein pools in live hNSC. In addition, the
HaloTag® protein continued to be expressed after hNSC differentiation
into neurons and astrocytes. Thus, the HaloTag® technology can be used
to study proteins of interest, and as such, represents a new research
tool to further reveal protein biology in stem cells.
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