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Maxwell® 16 Polyhistidine Protein Purification Kit: Automated Protein Purification with Maximum
Performance and Convenience
Natalie Betz, Ph.D., Cristopher Cowan, Ph.D.,
Steve Krueger, B.S.ChE, Susan Fly, B.S., Jude Stevens, B.S., and
Michael Bjerke, M.S.
Promega Corporation
The Maxwell® 16 Polyhistidine Protein Purification Kit allows simple,
convenient, and robust purification of polyhistidine-tagged proteins
from multiple sample types, including bacteria, mammalian cells, insect
cells, and culture medium. The system also purifies HQ-tagged proteins
from bacterial samples. The optimized automated method, compact
instrumentation, and pre-filled reagent cartridges maximize performance
while minimizing the hands-on time required for protein purification.
The purified protein is compatible with many common downstream
applications including polyacrylamide gel electrophoresis and detection,
functionality studies, Western blot analysis, and mass spectrometry. In
this article we demonstrate the performance of the Maxwell® 16 Polyhistidine Protein Purification Kit for low- to moderate-throughput
automated protein purification. Purification results with the Maxwell®
System are comparable to or better than those achieved using manual
processing methods.
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