|
Download
article
222kb
pdf? |
|
A Novel System for Regulated Protein Expression in Mammalian Cells
Jolanta Vidugiriene, Ph.D., Jim Hartnett, M.S.,
Elaine Schenborn, Ph.D., and Michael R. Slater, Ph.D.
Promega Corporation
We describe the Regulated Mammalian Expression System, which allows
researchers to achieve tightly controlled protein expression in
mammalian cells. This system uses coumerin-based compounds to modulate
regulation of gene expression while maintaining high levels of induction
over low levels of basal activity. In addition, expression of the gene
of interest can be turned off rapidly by novobiocin addition.
Introduction of a unique auto-amplification loop for transactivator
expression makes the system robust and minimizes potential undesired
effects on host gene expression.
|
