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TSAP: A New Thermosensitive Alkaline Phosphatase
John Brandon, M.A., and Michael R. Slater, Ph.D.
Promega Corporation
Here we describe TSAP, a new thermosensitive alkaline phosphatase. TSAP is
active in all Promega restriction enzyme buffers, including MULTI-CORE™
Buffer, as well as both GoTaq® Buffers. The robust phosphatase activity
effectively dephosphorylates all DNA termini (5´, 3´ and blunt) in 15
minutes at 37°C. As TSAP is active in restriction enzyme buffers, using
TSAP offers the potential for simultaneous restriction digestion/dephosphorylation.
Promega TSAP is thoroughly tested to ensure functionality and physical
purity.
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