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Functional Protein Production in the TNT® SP6
High-Yield Protein Expression System
Kate Qin Zhao, Ph.D., Robin Hurst, M.S., Sarah
Wheeler, B.S., Becky Godat, M.S., Jami English, M.S., Jim Hartnett,
M.S., and Michael Slater, Ph.D.
Promega Corporation
The wheat germ cell-free TNT® SP6 High-Yield Protein Expression
System produces high levels of functional protein. Functional protein
yield can reach 100µg/ml in batch mode and 400–500µg/ml in dialysis
mode. A method for the production of functional procaspase-3 and the
mature protein, caspase-3, using the same vector with different modes of
protein synthesis is described. Full-length procaspase-3 was produced
(>70µg/ml) in batch mode, while functional processed caspase-3 was
produced (~400–500µg/ml) in dialysis mode.
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